286 BACTERIOLOGY. 



bundles are spreading out like locks or plaits of 

 hair in the neighbouring gelatine. These appear- 

 ances are perfectly characteristic (Fig. 108). 



Cover-glass impressions. The plate-cultivations 

 should be also examined as soon as the colonies 

 appear, by making cover-glass impressions (p. 52), 

 and staining them with aniline dyes. The filaments, 

 examined with a high power, will then be seen to 

 consist of a number of rods or segments (Plate I., 

 3)' O n tne other hand, filaments from a 



FIG. 109. COVER GLASS IMPRESSION-PREPARATION, x 70. 



tube cultivation in a solid medium will be found 

 to be composed, not only of rods, but here and 

 there of torula-like involution- forms (Plate I., Fig. 

 30). In a cover-glass impression from a potato - 

 culture (Plate I., Fig. 29) the individual segments 

 have a great tendency to be isolated one from the 

 other, and there is copious spore-formation. 



Preservation of spores. Spores may be preserved 

 simply by allowing anthracic blood to dry and 

 sealing it in a tube. The spores from a potato 



