2Q 2 BACTERIOLOGY. 



Wash in alcohol, rinse in water, and transfer to picro- 

 carmine solution (Weigert) for from half an hour to an 

 hour. Treat with alcohol till the colour is almost washed 

 out, and finally clear in oil of cloves and mount in Canada 

 balsam. 



Bacillus mallei (Bacillus of glanders). Rods 

 about the size of tubercle bacilli (Fig 113). When 

 cultivated on solid sterile blood serum at 38 C., the 

 growth appears in the form of minute, transparent 

 drops consisting entirely of the characteristic bacilli. 

 On sterilised potatoes they form, in a week to ten 

 days at 37 C., a brown gelatinous layer. Pure cul- 



FIG. 113. BACILLUS MALLEI, x 1200; from a section of a glanders' nodule. 



i 



tivations after several generations produce the fol- 

 lowing results when inoculated into horses, rabbits, 

 guinea-pigs, and field-mice. A spreading ulcer with 

 indurated base appears at the site of inoculation, 

 while smaller ulcers break out in its vicinity. The 

 lymphatics become swollen, and general infection 

 follows in the form of nodules in the internal organs, 

 and nodules and ulcers on the nasal septum. In 

 guinea-pigs a characteristic tumour of the testis, or 

 ovary and vulva, frequently results, and should be 

 prepared for microscopical sections. The bacilli 

 are found in the nodules of the nasal mucous mem- 



