SYSTEMATIC AND DESCRIPTIVE. 2 93 



brane, the lung, spleen, liver, and other organs in 

 horses and sheep affected with glanders. 



METHODS OF STAINING THE BACILLUS OF GLANDERS. 



The bacilli of glanders are extremely difficult to demon- 

 strate. The most satisfactory results are obtained as 

 follows : 



Method of Schuts. The sections are placed for twenty- 

 four hours in a mixture ot 



Potash solution (i in 10,000); {Equal 



Concentrated alcoholic methylene-blue solution;] parts. 



Wash the sections in a watch-glass with water acidulated 

 with four drops of acetic acid. Transfer for five minutes 

 to 50 per cent, alcohol, fifteen minutes to absolute alcohol, 

 clarify in clove-oil, and mount in Canada balsam. 



Bacillus cedematis maligni, Koch (Pas- 

 teur's Septicczmid). Rods from 3 3*5 /* long and 

 i i 'i IL wide; they mostly lie in pairs, and then 

 appear to be double this length. The rods are 

 rounded at their ends, and form threads which are 

 sometimes straight, but more commonly curved. In 

 stained preparations they have a somewhat granular 

 appearance. The bacilli are distinguished from 

 anthrax bacilli by their being somewhat thinner, 

 by their rounded ends, and by their being motile. 

 Anthrax bacilli also never appear as threads in fresh 

 blood, and are differently distributed throughout 

 the body. They are anaerobic, and can be culti- 

 vated on blood-serum and on neutral solution of 

 Liebig's meat extract in an atmosphere of carbonic 



