CONTENTS. 



CHAPTER I. 

 GENERAL MORPHOLOGY AND BIOLOGY. 



PAGE 



Introductory Terminology Structure of the bacterial cell Reproduction 

 of bacteria Spore formation Motility Minuter structure of bacterial 

 protoplasm Chemical composition of bacteria Classification Food 

 supply Relation of bacteria to moisture, gaseous environment, tempera- 

 ture, and light Conditions affecting bacterial motility Effects of bac- 

 teria in nature Methods of bacterial action Variability among bacteria I 



CHAPTER II. 

 METHODS OF CULTIVATION OF BACTERIA. 



Introductory Methods of sterilisation The preparation of culture media 

 The use of the culture media The methods of the separation of aerobic 

 organisms The principles of the culture of anaerobic organisms Mis- 

 cellaneous methods General laboratory rules 27 



CHAPTER III.. 



MICROSCOPIC METHODS GENERAL BACTERIOLOGICAL DIAGNOSIS 

 INOCULATION OF ANIMALS. 



The microscope Examination of hanging-drop cultures Film preparations 



Examination of bacteria in tissues The cutting of sections Staining 

 principles Mordants and decolorisers Formulae of stains Gram's 

 method and its modifications Stain for tubercle and leprosy bacilli 

 Staining of spores and flagella Observation of agglutination and sedi- 

 mentation Routine bacteriological examination Methods of inoculation 



Autopsies on animals 87 



CHAPTER IV. 

 BACTERIA IN AIR, SOIL, AND WATER. ANTISEPTICS. 



AIR: Methods of examination Results. SOIL: Methods of examination 

 Varieties of bacteria in soil. WATER : Methods of examination Bacteria 

 in water Bacterial treatment of sewage. ANTISEPTICS : Methods of 

 investigation The action of antiseptics Certain particular antiseptics . 123 



