WRIGHT'S METHOD OF ANAEROBIC CULTURE. 



approximately equal bulk of pyrogallic acid), and adding to that 



i c.c. of a 5 percent solution of sodium hydrate. A rubber stopper 



is next tightly fitted into the end of the 



tube, and the apparatus is then ready 



for incubating. The second method, 



suitable for fluid media only, consists of 



a system of soft rubber and glass tubes 



arranged in an ordinary culture tube, as 



shown in Fig. 30. The small glass tube 



A is drawn out slightly at both ends 



and capped by 

 pieces of soft 

 rubber tubing, 

 into one of 

 which is in- 

 serted a short 

 length of glass 

 tubing of a 

 much smaller 

 calibre than A, 

 and which is 

 plugged with 

 cotton at its up- 

 per end, where 

 a small piece 



II I of rubber tub- 



Al IHfill ing is affixed. 



Into the tube 

 containing 



such an apparatus the usual quantity 

 of bouillon is poured, and the whole is 

 sterilised by steam, care being taken 

 that the rubber portions are not bent. 

 To prepare a culture it is advisable to 

 boil the broth over a free flame before 

 inoculating, so as to drive off as much 

 dissolved oxygen as possible, then im- 

 merse the tube in cold water. When 



cool, inoculate the bouillon and suck it up into the system of 



tubes well above C, then, pinching E between the fingers to 



By permission, from Mallory & 

 Wright's " Pathological Technique." 



FIG. 29. Wright's method 

 for the cultivation of anaerobes 

 in solid media. 



By permission, from Mallory & 

 Wright's " Pathological Technique." 



FIGS. 30, 31. Wright's 

 method for the cultivation of 

 anaerobes in flilid media. 



