INTRAPERITONEAL INOCULATION. 119 



has its opening, not at the point, but in the side in the middle of 

 the arch (Fig. 56). The hair over the lower part of the abdomen 

 is cut, and the skin purified with an antiseptic. The whole thick- 

 ness of the abdominal walls is then pinched up by an assistant, 

 between the forefingers and thumbs of the two 

 hands. The needle is then plunged through the 

 fold thus formed. The result is that the hole in 

 the side of the needle is within the abdominal 

 cavity, and the inoculation can thus be made. 

 Intraperitoneal inoculation can also be practised 

 with an ordinary needle. The mode of proced- 

 ure is similar, but after the needle is plunged 

 through the abdominal fold, it is partially with- 

 drawn till the point is felt to be free in the peri- FIG. 56. Hollow 

 toneal cavity, when the injection is made. There aperture^at^fon?- 

 is little risk of injuring the intestines by either trapedtoneai inocu- 



, i i lations. 



method. 



4. Intravenous Injection. The vein most usually chosen is 

 one of the auricular veins ; preferably the posterior lateral 

 branch. The part has the hair removed, the skin is purified, 

 and the vein made prominent by pressing on it between the 

 point of inoculation and the heart. The needle is then plunged 

 into the vein and the fluid injected. That it has perforated the 

 vessel will be shown by the escape of a little blood ; and that 

 the injection has taken place into the lumen of the vessel will 

 be known by the absence of the small swelling which occurs in 

 subcutaneous injections. If preferred, the vein may be first 

 laid bare by snipping the skin over it. The needle is then 

 introduced. 



5. Inoculation into the Anterior Chamber of the Eye. Local 

 anaesthesia is established by applying a few drops of 2 per cent 

 solution of hydrochlorate of cocaine. The eye is fixed by 

 pinching up the orbital conjunctiva with a pair of fine forceps, 

 and the edge of the cornea being perforated by the hypodermic 

 needle, the injection is easily accomplished. 



Sometimes inoculations are made by planting small pieces of 

 pathological tissues in the subcutaneous tissue. This is especially 

 done in the case of glanders and tubercle. The skin over the 

 back is purified, and the hair cut. A small incision is made with 

 a sterile knife, and the skin being separated from the subjacent 



