222 INFLAMMATORY AND SUPPURATIVE CONDITIONS. 



(2) By cultures, (a) FraenkeFs pneumococcus. With similar 

 material make successive strokes on agar, blood agar, or blood 

 serum, or by inoculation of milk tubes. The most certain 

 method, however, is to inject some of the material containing 

 the suspected cocci into a rabbit. If the pneumococcus be 

 present the animal will die, usually within forty-eight hours, wrth 

 numerous capsulated pneumococci in its heart blood. With the 

 latter inoculate tubes of the above media and observe the growth. 

 In some cases of severe pneumococcic infection the organism 

 may be cultivated from the blood obtained by venesection 

 (P- 73)- (b) Friedldnder 1 s pneumobacilhis can be readily isolated 

 either by ordinary agar and gelatin plates or by successive strokes 

 on agar media. 



