318 ANTHRAX. 



and successive strokes made on agar tubes, which are then 

 incubated at 37 C. At the end of twenty-four hours anthrax 

 colonies will appear, and can be readily recognised from their 

 wavy margins, by means of a hand lens. They should also be 

 examined microscopically by means of film preparations. 



(c) Test Inoculations. A little of the suspected material 

 should be mixed with some sterile bouillon or water, and injected 

 subcutaneously into a guinea-pig or mouse, or it may be intro- 

 duced into the subcutaneous tissue by means of a seton. If 

 anthrax bacilli are present, the animal usually dies within two- 

 days, with the changes in internal organs already described. 



