CONTENTS vii 



49. PREPARATION OF ASHBY'S SOLUTION 28 



50. PREPARATION OF NITROGEN-FREE MEDIA FOR CULTURE OF 



SYMBIOTIC NITROGEN-FIXING BACTERIA 28 



51. PREPARATION OF WINOGRADSKY AND OMELIANSKI'S SOLUTION 



FOR CULTIVATING NITRITE-FORMING ORGANISMS .... 29 



52. PREPARATION OF WINOGRADSKY AND OMELIANSKI'S SOLUTION 



FOR CULTIVATING NITRATE-FORMING ORGANISMS .... 29 



53. PREPARATION OF N^EGELI'S SOLUTION 29 



54. PREPARATION OF CZAPEK'S NUTRIENT SOLUTION FOR THE CUL- 



TIVATION OF FUNGI 30 



55. PREPARATION OF SULPHINDIGOTATE BOUILLON FOR DETECTING 



ANAEROBIC ORGANISMS 30 



METHODS OF CULTIVATING ANAEROBIC BACTERIA .... 30 



PREPARATION OF STERILE WATER BLANKS 32 



SECTION VII. FUNDAMENTAL METHODS USED IN THE 

 STAINING AND EXAMINATION OF BACTERIA 



58. PREPARATION OF SIMPLE STAINS 33 



59. GENERAL METHOD OF MAKING STAINED PREPARATIONS . . 34 



60. PREPARATION OF CONTRAST STAINS 35 



61. SPECIAL STAINS 36 



62. DIFFERENTIAL STAINING 37 



SECTION VIII. FUNDAMENTAL METHODS OF ISOLATION 



63. ISOLATION OF A PURE CULTURE 39 



SECTION IX. OUTLINE FOR THE ROUTINE CULTIVATION 

 OF BACTERIA 



64. PRELIMINARY EXAMINATION 41 



65. STUDY OF BACTERIAL CHARACTERS 41 



SECTION X. BACTERIA OF THE AIR 



66. RELATION OF BACTERIA TO ATMOSPHERIC DUST 48 



67. DETERMINATION OF THE NUMBER OF BACTERIA IN AIR . . 48 



68. STUDY OF MICROCOCCUS CANDICANS 50 



69. STUDY OF SARCINA LUTEA 50 



70. STUDY OF BACILLUS FLUORESCENT LIQUE FACIE NX 50 



71. STUDY OF BACILLUS PRODIGIOSUS 50 



72. STUDY OF BACILLUS SUDTILIS (HAY BACILLUS) 50 



