CONTENTS xi 



APPENDIX A. STERILIZATION 



STERILIZATION WITH CHEMICALS 115 



STERILIZATION WITH HEAT 117 



HOT-AIR STERILIZATION 117 



STEAM STERILIZATION AT 100 C 118 



STEAM STERILIZATION UNDER PRESSURE 119 



PARTIAL STERILIZATION PASTEURIZATION 120 



STERILIZATION BY FILTRATION 121 



STERILIZATION BY LIGHT 124 



THE TECHNIQUE OF STERILIZATION FOR SPECIAL PURPOSES 125 



GLASSWARE, PIPETTES, AND INSTRUMENTS 125 



LIQUID MEDIA AND OTHER SOLUTIONS . 125 



GELATIN AND AGAR MEDIA 126 



SUGAR-CONTAINING MEDIA 127 



SOLID VEGETABLES 127 



MILK 127 



BLOOD SERUM 128 



SOIL . . 128 



SEEDS 131 



GROWING HIGHER PLANTS UNDER STERILE CONDITIONS . 134 



APPENDIX B. HANDLING STOCK CULTURES 138 



APPENDIX C. MAKING PERMANENT PREPARATIONS 139 



APPENDIX D. THE INOCULATING CHAMBER 141 



APPENDIX E. THE TITRATION OF BOUILLON 142 



APPENDIX F. THE DETERMINATION OF AMMONIA 143 



APPENDIX G. THE DETERMINATION OF NITRATE 146 



APPENDIX H. THE DETERMINATION OF NITRITE 148 



APPENDIX I. THE DETERMINATION OF NITRITES BY TROMMSDORF'S 



METHOD 150 



APPENDIX J. DETERMINATION OF TOTAL NITROGEN .... 151 



APPENDIX K. THE DETERMINATION OF REDUCING SUGAKS . 154 



