APPENDIX P KIT 



3. Lbffler's blood serum 



Stroke, invisible, scanty, moderate, abundant. 



Form of growth, filiform, echinulatc, beaded, spreading, plumose, arbores- 

 cent, rhizoid. 



Elevation of growth, fiat, effuse, raised, convex. 

 Luster, glistening, dull, cretaceous. 

 Topography, smooth, contoured, rugose, verrucose. 



Chromogenesis (7), 



Medium, grayed, browned, reddened, blued, greened. 

 Liquefaction begins in days, complete in days. 



4. Agar stab 



Growth, uniform, best at top, best at bottom; surface growth scanty, abun- 

 dant ; restricted, widespread. 



Line of puncture, filiform, beaded, papillate, villous, plumose, arborescent ; 

 liquefaction. 



5. Gelatin stab 



Growth, uniform, best at top, best at bottom. 



Line of puncture, filiform, beaded, papillate, villous, plumose, arborescent. 



Liquefaction, crateriform, napiform, infundibuliform, saccate, stratiform ; 



begins in days, complete in days. 



Medium, fluorescent, browned 



6. Nutrient broth 



Surface growth, ring, pellicle, fiocculent, membranous, none. 



Clouding, slight, moderate, strong ; transient, persistent ; none ; fiuid turbid. 



Odor, absent, decided, resembling 



Sediment, compact, fiocculent, granular, flaky, viscid on agitation, abun- 

 dant, scant. 



7. Milk 



Clearing without coagulation. 

 Coagulation, prompt, delayed, absent . 



Extrusion of whey begins in days. 



Coagulum, slowly peptonized, rapidly peptonized. 



Peptonization begins on day, complete on day. 



lleaction, 1 day , 2 days , 4 days , 10 days , 



20 days 



Consistency, slimy, viscid, unchanged. 

 Medium, browned, reddened, blued, greened. 

 Lab ferment, present, absent. 



8. Litmus milk 



Acid, alkaline, acid then alkaline, no change. 



Prompt reduction, no reduction, partial slow reduction. 



9. Gelatin colonies 



Growth, slow, rapid. 



J?orm,punctiform, round, irregular, amoeboid, mycelioid, filamentous, rhizoid. 

 Elevation, fiat, effuse, raised, convex, pulvinate, crateriform (liquefying). 

 Edge, entire, undulate, lobate, erose, lacer ate, fimbriate, filamentous, fioccose, 



curled. 

 Liquefaction, cup, saucer, spreading. 



