t 605 ] 



animals inoculated by the above method, only three died after 

 inoculation. 



" Hill cattle. There is a great difference in the dose 

 of serum required for the inoculation of hill cattle, as 

 compared with that which is safe in plain animals. Notwith- 

 standing that the serum simultaneous method by itself has 

 not been found to be reliable in this particular breed, yet on 

 re-inoculating these animals with from i to 10 c. c. of virulent 

 blood during the seven or eight days following the injection 

 by the simultaneous method, protection has been brought 

 about and an active immunity conferred. 



" Serum alone. The use of serum alone causes no 

 reactionary fever and it affords immediate full immunity and 

 is very useful in the case of dairy animals and pregnant cows, 

 where it is desirable that the milk supply should not be 

 interfered with, and no cases of abortion take place. The 

 temporary immunity given by injecting with serum alone is 

 sufficient to protect the animals throughout an outbreak. 

 The experiments carried out at Mukhtesar proved that the 

 animals injected with 10, 20, 50. 100, and 150 c. c. per 600 

 Ibs. body weight, were found immune on the 43rd, 76th, io3rd 

 and i64th days, respectively, after serum injections, and in 

 each case when tested by the introduction of virulent blood 

 sub-cutaneously, only a slight temperature reaction followed, 

 clearly showing that the animals submitted to the above test 

 were perfectly protected and would remain so for a much 

 longer period than those stated above. 



" Manufacture of serum. During the year 1900, 464,765 

 C c. of protective rinderpest serum were produced at 

 Mukhtesar, and 119,880 c, c. have been issued to the 

 Veterinary Superintendents, etc, of different provinces in 

 India. At the present time we have 33,673 dt>ses tested and 

 ready for despatch on receipt of a telegram, but in addition 

 some 50,000 doses are already in stock and only require 

 testing, a matter of two weeks' duration. The manufacture 



