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the absence of better terms, we shall call the charbon symp- 

 tomatique of the French, simply charbon, and the charbon 

 virulent of the French, anthrax, to avoid confusion. 



1.321. Inoculation for protecting animals against disease 

 is done for both anthrax and charbon. The vaccines for 

 anthrax are prepared in different ways by different persons. 

 M. Pasteur's vaccine, however, is the one which is more 

 extensively used than any other, and the preparation of this 

 will be fully described. A description of two more recent 

 methods, viz., those of M. Chauveau, formerly Director of 

 the Veterinary College at Lyons, also applied in preparing 

 anthrax vaccine, will, however, be found both interesting and 

 useful, especially on account of their simplicity. M. Arloing, 

 the present Director, believes they are equally as effective as 

 Pasteur's method. 



1.322. The vaccine for charbon was first prepared by M. 

 Arloing. His system is now adopted in practice, and the 

 vaccine is sold by MM. Marais et Fromage, droguistes, Rue 

 Lebrim, a Paris. 



1.323. The characters that distinguish anthrax from 

 charbon are : 



(i). Anthrax is fatal to almost all animals known. Char- 

 bon virus, if inoculated into horses, mules, rabbits, or dogs, 

 does not have any effect. White mice may or may not die 

 of charbon when inoculated with the virus. The animals 

 most susceptible to charbon are cattle. Cows and oxen die 

 more frequently of charbon than of anthrax from which about 

 half the number recover. Sheep and guinea-pigs are also sus- 

 ceptible animals, that is invariably die when inoculated with 

 the virus, whether of charbon or of anthrax. The subject has 

 not yet been sufficiently investigated to enable one to say 

 whether man is more subject to one than to the other. 

 Limping before death is an invariable symptom in charbon. 



(2). (Edema takes place at the point of inoculation in 

 both anthrax and charbon. When an incision is made of the 



