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three grammes of the dust to every six grammes of water. 

 This mixture is placed in shallow porcelain capsules, each 

 capsule containing three grammes of the virulent dust and 

 six grammes of water. These capsules are placed inside 

 an etuve Arsonval, only four capsules being placed in one 

 etuve, lest the evaporated moisture should lower the tempera- 

 ture inside the etuve to any considerable extent. 



1.341. The stove used for attenuating the charbon virus is 

 not the ordinary etuve Arsonval used in Pasteur's laboratory. 

 The temperature at which the stove is to be adjusted is, 

 in the case of the premier vaccine 104 C, and in the case 

 oj the deuxieme 90 C. The jacket of the etuve thus must 

 not be filled with water, as water will begin to boil at 100 C. 

 A substance with a higher boiling point should therefore 

 be used in rilling up the jackets on the side and the top. 

 Any ordinary oil serves this purpose. The capsules are 

 kept for six hours inside the etuve both in the case of the 

 premier vaccine and in that of the deuxieme vaccine, after 

 which the contents of the capsules are found to be quite 

 dry. These are scraped out, well powdered, and stored 

 in dry bottles and in dry places as premier or deuxieme 

 vaccine, as the case may be. A few grains of this 

 powder well mixed with water is syringed into an animal 

 to protect it from charbon in the locality where this disease 

 is known to take place. The premier vaccine, which 

 is weaker, is inoculated first, and after a few days the 

 deuxieme. 



1.342. To regulate the etuves at a regular temperature of 

 104 C. in the one case and 90 C. in the other, a different 

 automatic appliance is employed from that used in Pasteur's 

 laboratory. The contrivance used by M, Arloing consists of 

 a tube, closed at the lower end, and containing mercury and 

 water, placed in a vessel of hot oil. The water used is pure 

 water in the case of the etuve for the deuxieme vaccine, 

 i.e., where a temperature of 90 C. is sufficient. In the case 



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