t 779 ] 



has elapsed the vaccine (still virulent) is sucked into sterilized 

 tubes, sealed at one end and having a cotton wool plug at 

 the other. The sealed end is broken off, the tube passed 

 through a flame four or five times with a twisting motion of 

 the wrist, dipped into the flask, and a portion of the vaccine 

 drawn in by sucking through the other end. When a suffi- 

 cient quantity has been drawn in the end is again sealed. 

 The cotton-wool end is also sealed beyond the cotton-wool, 

 so as to obtain a sealed glass tube containing virulent vaccine. 

 Several of these tubes are taken at the same time, placed on 

 a rack. This rack is plunged in a vessel containing water 

 kept by a similar automatic arrangement as has been already 

 described, at a uniform temperature of 48 C. for three hours. 

 At the expiration of this period of time the vaccine in the 

 tubes is sufficiently attenuated for application in the usual 

 manner to animals. This is the simplest and the quickest 

 way of preparing anthrax vaccine. 



1.345. The other method applied by M. Arloing in pre- 

 paring anthrax vaccine may be described as follows: 



1.346. Virulent bacillus anthracis is obtained from an 

 animal dying of anthrax in the usual way, and cultivated in 

 broth in a sterilized flask. This flask is put inside an iron 

 receiver, which is fitted on to a force pump, and oxygen, 

 prepared in the ordinary way and kept in an air-tight India- 

 rubber bag, is forced with this pump into the receiver. 

 When the manometre on the receiver indicates from 2 to 2j, 

 i. e. } when the pressure inside is 2 to 2\ atmospheres, the 

 screws are turned and the charged receiver is put inside an 

 etuve Pasteur for 14 to 20 days at a uniform temperature 

 of 36 C. After the expiration of this time the attenuation 

 of the vaccine is complete in the flask. But as only a small 

 quantity is contained in the flask, it is sown in a large 

 sterilized flask containing proved broth to increase the 

 quantity. The flask is taken out of the oxygen vessel and 

 its contents transferred to the large flask containing a large 



