t 782 ] 



*' seed/' or prepared locally, it will be necessary to have a 

 laboratory. And also to have, 



(1) Blowpipe flames, for making vaccine tubes, tubes 

 effiles and pipettes, and for sealing broth flasks, tubes, &c., 

 when necessary. 



(2) A refrigerator, to preserve the vaccine seed through- 

 out the year, unless the laboratory itself is situated in 

 a hill station. Premier vaccine keeps for two years, and 

 deuxieme for one year without degenerating or losing their 

 strength, if they are kept at a very low temperature. The 

 vaccine also, when it has been made, is kept in a cool place 

 below 23C., and this temperature cannot be naturally obtain- 

 ed in the plains in India. From this it will appear that it will 

 be probably impossible to carry on vaccination operations in 

 India during any other season than winter. In Europe it is 

 done only during the spring. 



(3) An Etuve Pasteur for the cultivation of the premier 

 vaccine, and another for that of the deuxieme. Different shelves 

 of the same etuve will answer if only a small number of flasks 

 have to be cultivated. In Pasteur's laboratory a whole room 

 is converted into an etuve, and one portion of it is used for the 

 premier vaccine only, and another for the deuxieme. But as a 

 difference has to be made between the " new" and "old " 

 *>., the filamentous vaccine, which is more useful, and which 

 is obtained during the first days of cultivation, and the 

 sporular vaccine, which is obtained if the cultivation is 

 allowed to proceed for ten days, and which is mixed with 

 filamentous vaccine if the vaccine has to be sent packed 

 up to some distance it is best to have two 6tuves to avoid 

 confusion. 



1,353. The Etuve Pasteur consists of (i) a shelved 

 chamber, with double boards and double glass doors, the 

 lowest shelf being covered up, communicating with the 

 upper board by means of several holes, and containing inside 

 jt a worm or coil filled with distilled or boiled water ; (2) a gas 



