to remain in the etuve and the vegetation to proceed, as it is 

 useful to mix a little " ancien " or sporular vaccine with the 

 more effective filamentous vaccine when vaccine is sent 

 tightly packed up to a distance. This same remark holds 

 good for premier as for deuxieme vaccine. But as the 

 deuxieme vaccine is much stronger than the premier, i.e., 

 capable of killing an animal not previously inoculated (only 

 about twelve days before) with the premier vaccine, it is very 

 important to keep the two entirely separate from each 

 other, and hence the necessity of two etuves 



(4) An Etuve Arsonval, for attenuating the virus for the 

 first time. As a constant higher temperature (42 C.) is 

 necessary for preparing the seed of the vaccine than that 

 required for cultivation of the vaccine (28 to 32 C), a sepa- 

 rate apparatus is necessary for this purpose. The Etuve 

 Arsonval already described in connection with the prepara- 

 tion of charbon vaccine is the apparatus used, with this differ- 

 ence only that hot water is put in the jacket instead of hot 

 oil, and the indicator is a glass tube exactly similar in arrange- 

 ment to that in the e*tuve Pasteur. 



(5) A four-d-flamber or a flame furnace. This is a 

 sheet-iron cylindrical stove with three jackets standing on a 

 gas burner. It is used for sterilizing (a) matras Pasteurs, or 

 vaccine flasks and seed flasks; (b) ballon pipettes^ or large 

 pipettes, used for transferring broth from sealed flasks to the 

 matras, and also for filling vaccine tubes ; (c) tube effiles^ 

 which are used for sowing seed in chicken broth first, and 

 also for sowing the vaccine in veal broth for increasing the 

 quantity ; (d) vaccine tubes, which are used for sending vac- 

 cine to a distance. 



(6) " Sulphate of copper vat, the solution being made with 

 one of sulphate of copper to 100 of water. In this is thrown 

 all the dead bodies of animals dying of anthrax, &c., after 

 experimental inoculation. 



d (7) A fold for larger animals, and a room with bx>xes of 



