t 785 1 



small animals, such as rabbits, guinea-pigs, goats, &c., to be 

 used for experiments. 



(8) A microscope magnifying 500 diameters, slides, cover 

 glasses, and a basin of sulphate of copper solution for putting 

 aside all microscopic preparations before washing. 



vg) Stoves for making broths, weighing machine, tubes, 

 matras, ballon pipettes, cotton-wool (for plugging matras, 

 pipettes, &c., before putting them in the stove for sterilizing), 

 India-rubber stoppers for vaccine tubes, spirit lamp, potash 

 for washing slides and cover glasses. 



(10) An auto-clave for clarifying and sterilizing broth. 



The operations necessary follow each other in the follow- 

 ing order : 



(1) Sterilizing the glasses with cotton-wool plugs, and 

 keeping a stock ready sterilized of each kind. 



(2) Preparing the veal and chicken broths, and having a 

 stock always ready in sealed flasks. 



(3) The sealed flasks are opened by rasping their neck 

 with a steel knife and cracking them off with an ignited piece 

 of Berzilius charcoal. A sterilized ballon pipette is taken, 

 the smaller sealed end nipped off, passed through flame, in- 

 troduced into the broth, the broth sucked into the pipette and 

 poured into the matras, veal broth being poured into the 

 larger flasks for the preparation of vaccine, and chicken broth 

 into the smaller matras for sowing seed. The matras are 

 left from 8 to 15 days in the lowest shelf of the Etuve Pasteur 

 to prove their sterility, in case any germ might have found its 

 way in any of the flasks while the pouring of the broth was 

 being done. The flasks or matras of broth are then brought 

 out and kept in a cool place to prevent evaporation and oxi- 

 dation to any extent. This stock can be used for sowing 

 seed or vaccine for 20 days to one month. After that the 

 broth gets too strong, being reduced in bulk by evaporation, 

 and it also becomes oxidized, and therefore a fresh supply must 

 be drawn from the sealed flasks again, or broth newly made. 



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