process proceeds, and spores are formed. This sporular state 

 or " seed'' of the premier and of the deuxieme vaccines can 

 be preserved in sealed tubes for over one year. At the be- 

 ginning of the season, therefore, the seeds of the virulent, pre- 

 mier, and deuxieme vaccines are obtained, and they are 

 stowed away in sealed flasks in a refrigerator at as low a 

 temperature as possible (o to 15 C.). 



(7) The sealed tubes containing the premier, the deu- 

 xieme, or the virulent vaccine is opened by nipping of the 

 wider end, the seed sucked into a small pipette or tube effile } 

 and sown into chicken broth, three flasks for every tube of 

 seed. They are left for three days at 30 to 32 C. in the 

 Etuve Pasteur, and then brought out and kept in a cool place. 

 They can be used for 15 days after they are taken out /. ^., 

 five days are allowed for each flask. 



(8) During these 15 days the contents of the three small 

 flasks can be sown into large flasks containing veal broth. 

 These large flasks are also left for three days as premier 

 or deuxieme vaccine in the Etuve Pasteur for culture, after 

 which it can be used for inoculating. 



(9) For despatch, a small quantity of vaccine (sporular or 

 ancien vaccine), kept 10 days in the etuve, is mixed with 

 the -fresh or filamentous vaccine in the ballon pipette before 

 introducing it into a vaccine tube and putting the India- 

 rubber cork on it. The vaccine tubes being all sterilized with 

 brown cotton wool plugs, these plugs are taken out, the vac- 

 cine poured into the tubes by a twist of the ballon pipette, an 

 assistant passing India-rubber stoppers at the same time 

 through the flame, taking the tubes one after another just 

 filled into his hands, and putting the plugs in with a 

 corkscrew motion. 



(10) Performing of the vaccination, first with the premier, 

 then with the deuxieme, and then for experiment only on a 

 few of the animals with the virulent vaccines. The Pravaz 

 syringe is used for this purpose, the leather rings of which 



