I 792 ] 



off in milk when they are vaccinated, and it is good to vac- 

 cinate them when they are dry. The milk from cows recently 

 vaccinated must be boiled before it is drunk. If malady does 

 not exist during the suckling period it is best to wait until 

 the weaning. Sheep are vaccinated in the inner part of 

 the thigh, the premier on the right thigh, and the deuxieme 

 on the left : oxen and horses behind the shoulder, sometimes 

 before, lest the collar should press on the place of inocula- 

 tion. It is well to shave off the hair of the part before 

 inoculating, lest the orifice of the syringe should be blocked 

 up with a hair and prevent inoculation. When a vaccine 

 tube has been uncorked once it must not be used a second 

 time -, what remains over after the first series of operations 

 must be rejected, and not reserved for another place or 

 occasion. 



1.359. Preparation of the Broths. For the prepara- 

 tion of anthrax vaccine, the best nutrient fluid to use is 

 chicken broth ; but, as it is dearer than veal broth, 

 which is also very good, chicken broth is used only 

 for sowing "seeds," or the sporular condition of the 

 virulent, deuxieme, and premier vaccines. In both cases, 

 however, the same proportion of meat and water is used, 

 viz., 250 grammes of meat to 500 grammes of water. 



1.360. Bones and fat are rejected, the meat is then 

 minced or triturated by passing it through a machine (the 

 one used at M. Duclaux's laboratory being constructed by 

 Messrs. Spong & Co., of London). This machine, somewhat 

 like a small turnip cutter, is fitted on to the corner of a 

 table. A weighing machine is also on the same table to 

 weigh the meat and the water. The meat and the water 

 are then put together in a glass vessel, covered up and laid 

 aside for 24 hours in the cold weather, and 12 hours at 

 night in the hot. After the 12 or 24 hours the mixture is 

 boiled for half an hour with continual stirring, and passed 

 through a calico filter, and then through a filter paper into 



