HO BACTERIOLOGY 



glycerin, macerated, filtered, and preserved in sealed 

 capillary glass tubes, which are broken and their contents 

 ejected by means of a small rubber bulb when used. 



Administration. The skin of the forearm or thigh is 

 thoroughly cleansed with soap and water, followed by al- 

 cohol. All traces, however, of alcohol must be removed 

 with sterile water before the vaccine is applied. Having 

 thoroughly cleansed the part, a small abrasion is made 

 either with a small needle or scarifier. This abrasion 

 should be just sufficient to cause an exudation of lymph, 

 care being taken to avoid drawing blood, which would 

 dilute the virus and wash it away, thus preventing a 

 successful vaccination. The virus is then ejected 

 from the tube upon the abrasion, thoroughly rubbed 

 in with the needle or scarifier, and allowed to dry in the 

 air, after which the spot should be protected by a shield 

 or large bunion pad, which protects it from rubbing or 

 injury, but at the same time permits a free circulation of 

 air to the part. 



Effects. In from five to eight days, if the inoculation 

 be successful, the part will become inflamed. There 

 will occur considerable swelling, with pain and more or 

 less swelling and tenderness of the adjacent lymphatic 

 glands. Some fever and general depression are also 

 present. At the site of inoculation there will occur 

 from one to five or six vesicles, which rapidly coalesce 

 and become pustular. These vesicles are umbilicated 

 and resemble small-pox pustules in a minute form. The 



