VACCINE OR OPSONIC THERAPY III 



vesic\e dries in from ten to twelve days and the scab 

 slips off, leaving a typic pitted scar, which is evidence of 

 successful vaccination. 



Rabies Vaccine (Pasteur Vaccine). Pasteur found 

 that the infective agent or its toxin in rabies was located 

 largely in the spinal cord of animals having the disease, 

 and that symptoms of the disease were produced in 

 healthy animals by the introduction of portions of the 

 spinal cord of animals having the disease. He also 

 found that the virulence of the poison was diminished 

 by drying. The longer the infective material was dried, 

 the less its virulence. He then determined that im- 

 munity to the disease could be produced by injection 

 of emulsions of the cord containing the infective agent, 

 beginning with a minute dose of the relatively non- 

 virulent material and gradually increasing the dose and 

 virulence of the injected material. 



Establishments termed Pasteur institutes are located 

 in all large cities, where the treatment may be carried 

 out. The vaccine is prepared by inoculating rabbits 

 beneath the dura mater with rabic material. The 

 animal develops rabies in six days, when the spinal cord 

 is removed and dried at a temperature of 68 F. 



A piece of the cord which has been dried for fourteen 

 days is mixed with sterilized veal broth and injected 

 beneath the skin of the abdomen. On the following day 

 material from a cord thirteen days' old is used, and so 

 on, each day using material a day younger, until fifteen 



