278 PYOGENIC BACTERIA. 



around the point of inoculation after a period of incubation of from 

 fifteen to sixty hours. This was attended with chilly sensations and 

 an elevation of temperature. Persons who had recently recovered 

 from an attack of erysipelas proved to be immune. 



Sections made from the ear of an inoculated rabbit, or of skin taken 

 from the affected area in erysipelas in man, show the streptococci in 

 considerable numbers in the lymph channels, but not in the blood 

 vessels. They are more numerous, according to Koch and to Fehl- 

 eisen, upon the margins of the erysipelatous area, and may even be 

 seen in the lymph channels a little beyond the red margin which 

 marks the line of progress of the infection. 



The researches of Weichselbaum and others show that Strepto- 

 coccus pyogenes is the infecting microorganism in a certain propor- 

 tion of the cases of ulcerative endocarditis. The author named 

 found it in four cases out of fifteen examined, and in two cases of 

 endocarditis verrucosa out of thirteen. In a previously reported series 

 of sixteen cases (fourteen of ulcerative endocarditis and two of ver- 

 rucosa) the streptococcus was found in six. 



In diphtheritic false membranes this streptococcus is very com- 

 monly present, and in certain cases attended with a diphtheritic exu- 

 dation, in which the Bacillus diphtherias has not been found by com- 

 petent bacteriologists, it seems probable that Streptococcus pyogenes 

 is the pathogenic microorganism responsible for the local inflamma- 

 tion and its results. Thus in a series of twenty-four cases studied by 

 Prudden in 1889 the bacillus of Loffler was not found, "but a strep- 

 tococcus apparently identical with Streptococcus pyogenes was found 

 in twenty-two." Chantemesse and Widal have also reported cases 

 in which a fibrinous exudate resembling that of diphtheria was as- 

 sociated with a streptococcus. " These forms of so-called diphtheria 

 are most commonly associated with scarlatina and measles, erysipe- 

 las, and phlegmonous inflammation, or occur in individuals exposed 

 to these diseases ; but whether exclusively under these conditions is 

 not yet established" (Prudden). 



Loffler has described under the name of Streptococcus articu- 

 lorurn a micrococcus obtained by him from the affected mucous 

 membrane in cases of diphtheria, and which he believes to be acci- 

 dentally present and without any etiological import in this disease. 

 In its characters it closely resembles Streptococcus pyogenes and is 

 perhaps a variety of this widely distributed species. Its characters 

 are described by Fliigge as follows : 



" Cultivated in nutrient gelatin, it forms at the end of three days small, 

 transparent, light-gray drops, upon the margin of which, under the micro- 

 scope, the cocci in twisted chains may be observed. As many as one hun- 



