286 PYOGENIC BACTERIA. 



has since recommended a mixture of nutrient agar (two parts), lique- 

 fied at a temperature of 50 C., with blood serum (two to three 

 parts) at 20 C. By quickly mixing with this a little pus .containing 

 the gonococcus he was able to obtain colonies upon plate cultures, 

 made by pouring the liquid medium upon sterile glass plates in the 

 usual manner. 



Development does not occur below 25 or above 38 C. The 

 writer has shown that a temperature of 60 C. maintained for ten 

 minutes destroys the infective virulence of gonorrhoeal pus. 



Pathogenesis. That the gonococcus is the cause of the specific 

 inflammation and purulent discharge characteristic of gonorrhoea is 

 now generally admitted upon the experimental evidence obtained by 

 Bumm. Having succeeded in obtaining it in pure cultures from 

 gonorrhoeal pus, he made successful inoculations in the healthy ure- 

 thra in two cases once with a third culture and once with one 

 which had been transferred through twenty successive generations. 



FIG. 87. Gonorrhceal conjunctivitis, second day of sickness; section through the mucous mem- 

 brane of upper eyelid; invasion of the epithelial layer by gonococci. (Bumm.) 



In both cases a typical gonorrhoea developed as a result of the inocu- 

 lation. 



Schrotter and Winkler (1890) report their success in cultivating 

 the gonococcus upon albumin from the egg of the pewit " Kibitz.'' 

 In the culture oven at 38 C. a thin, transparent, whitish layer was 

 already visible at the end of six hours and rapidly extended ; the 

 growth was less abundant at the end of three days, and had entirely 

 ceased by the fifth day. Attempts to cultivate the same microor- 

 ganism in albumin from hens' eggs gave a negative result. 



Aufuso (1891) has cultivated the gonococcus in fluid obtained 

 from the knee joint in a case of chronic synovitis, but failed to culti- 

 vate it in the fluid of ascites. A culture of the twelfth generation 

 made upon the culture medium mentioned, solidified by heat, was 

 introduced into the urethra of a healthy man and gave rise to a 

 characteristic attack of gonorrhoea. 



The mucous membranes in man which are subject to gonorrhoeal 

 infection are those of the urethra, the conjunctiva, the cervix uteri, 



