THE BACILLUS OF TYPHOID FEVER. 355 



media containing sugar grape sugar, cane sugar, or milk sugar. 

 The medium recommended by Smith for making this test is a pep- 

 tone-bouillon containing two per cent of grape sugar and made 

 slightly alkaline with carbonate of soda. The liquid becomes clouded 

 throughout at the end of twenty-four hours, but not a trace of gas is 

 developed even after several days. On the other hand, the colon 

 bacillus and other bacilli which closely resemble the typhoid bacillus 

 cause an abundant development of gas in this medium. 



PLATE V. 



PATHOGENIC BACTERIA. 



FlG. 1. Bacillus authracis from cellular tissue of inoculated mouse. 

 Stained with gentian violet, x 1,000. Photomicrograph by Frankel and 

 Pfeiffer. 



FIG. 2. Bacillus anthracis in section of liver of inoculated rabbit. 

 Stained with Bismarck brown, x 250. Photomicrograph by Sternberg. 



FlG. 3. Micrococcus gonorrhceae in gonorrhoeal pus. Stained with gen* 

 tiaii violet, x 1,000. Photomicrograph by gaslight (Sternberg). 



FlG. 4. Anthrax spores from a bouillon culture. Double-stained prepara- 

 tion with carbol-fuchsin and methylene blue, x 1,000. Photomicrograph 

 by Frankel and Pfeiffer. 



FIG. 5. Spirillum cholerse Asiaticae from a culture upon starched linen 

 at end of twenty-four hours Stained with fuchsin. x 1,000. Photomi- 

 crograph by Frankel and Pfeiffer. 



FIG. 6. Bacillus diphtherias from colony upon an agar plate, twenty- 

 four hours old. Stained with Loffler's solution of methylene blue, x 1,000. 

 Photomicrograph by Frankel and Pfeiffer. 



