400 



BACILLI IN CHRONIC INFECTIOUS DISEASES. 



associated with various other bacteria which grow more readily in 

 our culture media, it is not easy to obtain pure cultures, by the plate 

 method, from such material. 



In the guinea-pig subcutaneous inoculation is followed in four or 

 five days by tumefaction at the point of inoculation, and after a time 

 a prominent tumor with caseous contents is developed ; ulceration of 

 the skin follows, and a chronic, purulent ulcer with irregular, indu- 

 rated margins results ; after a time this may cicatrize. Meanwhile 

 the lymphatic glands become involved, and the symptoms of general 



Fio. 125. Section through a glanders nodule in liver of field mouse. Tissue X 250. Bacilli 

 X 500. (Baumgarten.) 



infection are developed at the end of four or five weeks ; the glands 

 suppurate, and in males the testicles are also involved ; finally a dif- 

 fuse inflammation of the joints occurs, and death results from ex- 

 haustion. In the guinea-pig the specific ulcers upon the nasal mu- 

 cous membrane, which characterize the disease in the horse, are rarely 

 developed to any great extent. 



In field mice general infection occurs at once as a result of the 

 subcutaneous injection of a small quantity of a pure culture, and the 

 animal dies at the end of three or four days. Upon post-mortem 



