44(> PATHOGENIC AEROBIC BACILLI 



BACILLUS h OF BOOKER. 



' ' Found in one case of mild dysentery, not in large quantity. 



" Morphology. Resembles Bacterium coli commune. 



" Growth in Colonies. Gelatin: In plain neutral gelatin the colonies re- 

 semble those of Bacterium coli commune. In sugar gelatin the colonies are 

 white and spread exten'sively. Slightly magnified, they have a round, dark 

 centre surrounded by a yellow, loose zone with an outer white rim ; later 

 the whole colony has a uniform yellow color and is not compact. 



' ' Agar : Colonies are white, round, and large. Slightly magnified, they 

 are brownish-yellow. 



" Stab Cultures. Nothing characteristic in gelatin and agar. 



"Potato culture is yellow, dry, and slightly raised, with well-defined bor- 

 ders. 



" Action on Milk and Litmus Reaction. Milk is coagulated into a solid 

 clot in two days at 38 C. Milk colored blue with litmus is changed to pink 

 in twenty-four hours. 



" Gas Production. Occurs in milk; not observed on potato. 



"Relation to Gelatin. Does not liquefy gelatin." 



BACILLUS k OF BOOKER, 



" Found in two cases of cholera infantum and one of catarrhal enteritis. 



" Morphology. Resembles Bacterium coli commune. 



' ' Growth in Colonies. Gelatin : In neutral gelatin the colonies cannot be 

 distinguished from those of Bacterium coli commune. In acid gelatin the 

 colonies do not spread so extensively as those of Bacterium coli commune, 

 and they have a decided concentric arrangement, a wide white centre sur- 

 rounded by a narrow, transparent blue ring, and outside of this a white bor- 

 der. Slightly magnified, the colonies have an irregular, yellowish-brown 

 centre mottled over with dark spots and surrounded by a light-yellow ring 

 bordered by a brownish-yellow wreath. 



"Agar: Colonies are large,round, and bluish-white. Slightly magnified, 

 a light brownish-yellow color. 



" Stab Cultures. Gelatin: In sugar gelatin the surface growth is exten- 

 sive, nearly colorless, and has a rough, misty appearance. In the depth if a 

 delicate growth. In plain neutral gelatin the surface growth is bluish -white, 

 thick, and not so extensively spread ; the growth in the depth is also thicker. 



' ' Potato culture is moist, dirty-cream color, has raised surface and defined 

 border. 



" Action on Milk. Milk becomes gelatinous in twenty-four hours at 38 

 C., and a solid clot in two days. Milk colored blue with litmus is changed to 

 pink in twenty- four hours, and reduced to white with a pink layer on top in 

 two days," 



BACILLUS H OF BOOKER, 



' ' Found in large quantity, but not the predominating form, in one case 

 of chronic gastro-enteric catarrh extremely emaciated. 



" Morphology. Resembles Bacterium coli commune. 



" Growth in Colonies. Gelatin : In neutral gelatin the colonies are spread 

 out and have a frosty or ground-glass appearance. The centre is blue and 

 border white, but both have the ground-glass appearance. Slightly magni- 

 fied, the central part is light yellow and the border brown with a rough, fur- 

 rowed surface. In acid gelatin the white border is wider and the surface is 

 rougher. 



'* Agar: Colonies are round, blue, or bluish- white, and spread out. Under 

 the microscope they have a light-yellow color. 



"Stab Cultures. Gelatin: Has a rough, nearly colorless surface growth, 

 and a thick stalk in the depth along the line of inoculation. 



' ' Agar : Thick white surface growth with well-developed stalk in the depth. 



