466 PATHOGENIC AEROBIC BACILLI 



and a small spleen. In mice no oedema and a slightly enlarged spleen. The 

 bacilli are found in the blood of the heart in small numbers, and are some- 

 what more numerous in the spleen, especially in mice. 



109. BACILLUS OF LETZERICH. 



Obtained by Letzerich (1887) from the urine of children suffering from 

 "nephritis interstitialis primaria." Etiological relation not satisfactorily 

 demonstrated. 



Morphology. Bacilli with round ends, straight or slightly curved, often 

 forming filaments. 



Stains with the usual aniline colors. 



Biological Characters. An aerobic, liquefying bacillus. Forms spores. 

 Grows rapidly in nutrient gelatin at a comparatively low temperature best 

 at 14 C. Upon gelatin plates, at 14 C., complete liquefaction has occurred 

 in from thirty-six to forty-eight hours, and a thin, white film covers the 

 surface of the liquefied gelatin; the same in gelatin stick cultures. 



Pathogenesis. Rabbits injected in the cavity of the abdomen are said to 

 die in about fourteen days. The autopsy shows an extensive abscess, en- 

 largement and congestion of the kidneys, enlarged spleen, etc. The bacilli 

 are found in great numbers in all of the organs. 



110. BACILLUS OF SCHIMMELBUSCH. 



Obtained by Schimmelbusch (1889) from the necrotic tissues at the boun- 

 dary line of the still living tissues in cancrum oris, or noma. Etiological 

 relation not proved. 



Morphology. Small bacilli with round ends; often united in pairs; 

 may grow out into long filaments. 



Stains best with an aqueous solution of gentian violet ; does not stain by 

 Gram's method. 



Biological Characters. An aerobic, non- liquefy ing bacillus. Grows in 

 the usual culture media at the room temperature better in the incubating 

 oven at 30 to 37 C. Upon gelatin plates forms below the surface spheri- 

 cal, finely granular, grayish- white colonies, which come to the surface and 

 form elevated masses with slightly dentate margins and an irregularly cleft 

 surface. In gelatin stick cultures the growth along the line of inoculation 

 is coarsely granular ; upon the surface a broad, flat layer. Upon the sur- 

 face of agar, in twenty -four hours at 37 C., a grayish- white layer along the 

 line of inoculation, which is smooth and about three millimetres in breadth. 

 Upon potato, at the end of two weeks, a broad, moist, grayish- white layer 

 from two to three millimetres wide. Upon coagulated ascitic fluid, at the 

 end of twenty-four hours, a thin layer along the impfstrich, from, which 

 lateral offshoots are given off. 



Pathogenesis. Cultures injected subcutaneously into rabbits produced 

 local abscesses only ; not pathogenic for mice or pigeons. 



111. BACILLUS FCETIDUS OZJENM. 



Obtained by Hajek (1888) from the nasal secretions of patients with ozae- 

 na. Etiological relation not proved. 



Morphology. Short bacilli, but little longer than broad; usually in pairs, 

 or in chains of six to ten elements. 



Stains with Loffler's solution of methylene blue or solutions of aniline 

 colors in aniline water not so well in aqueous solutions ; does not stain by 

 Gram's method. 



Biological Characters. An aerobic and facultative anaerobic, liquefy- 

 ing, motile bacillus. Spore formation not observed. Grows in the usual 

 culture media at the room temperature. Upon gelatin plates the colonies, 

 at the end of thirty-six hours, are scarcely visible, with well-defined but 



