470 PATHOGENIC AEROBIC BACILLI 



deposit accumulates at the bottom of the tube. Upon the surface of agar 

 whitish colonies are formed, which coalesce to form a thin layer. Upon 

 potato a tolerably thick, somewhat iridescent, brown layer is formed, which 

 extends over the entire surface. In bouillon, at the end of twenty-four to 

 forty-eight hours at 37 C., a greenish-yellow color is developed, first near 

 the surface and later extending throughout the fluid, which acquires the color 

 of a dilute solution of sulphate of copper ; a whitish film forms upon the 

 surface. In anaerobic cultures the color is a pale brown instead of green. 



Pathogenesis. Rabbits die at the end of two to three weeks after receiv- 

 ing an intravenous injection. At the autopsy they are found to have pneu- 

 monia with pleuritis and pericarditis. 



135. BACILLUS CANALIS CAPSULATUS. 



Obtained by Mori (1888) from sewer water. 



Morphology. Bacilli with round ends, elliptical or rod-shape in form, 

 and from 0.9 to 1.6 /j. thick ; often surrounded with a broad capsule, which 

 is always seen in preparations from the blood or tissues of an infected ani- 

 mal ; sometimes in pairs with the acute ends of the rods in apposition, and 

 surrounded by a single capsule. 



Stains with the usual aniline colors, but not by Gram's method. 



Biological Characters. An aerobic and facultative anaerobic, non- 

 liquefying, non-motile bacillus. Spore formation not observed. Grows in 

 the usual culture media at the room temperature. Upon gelatin plates 

 hemispherical, porcelain-white, sharply defined colonies, resembling those of 

 Friedlander's bacillus, are developed at the end of twenty -four hours. In 

 gelatin stick cultures development occurs along the line of puncture and 

 upon the surface, forming a " nail-shaped " growth similar to that of Fried- 

 lander's bacillus (Bacillus pneumonias) in the same medium. Upon agar 

 a viscid and abundant growth is formed in the incubating oven at 37 C. 

 Upon potato an abundant development in the form of a yellowish, moist, vis- 

 cid layer, with irregular outlines. In bouillon, at the end of three or four 

 days, a white film forms on the surface, especially in contact with the test 

 tube. 



Pathogenesis. Mice die in two to three days after receiving a subcutane- 

 ous injection. Guinea-pigs and rabbits are immune. 



136. BACILLUS CANALIS PARVUS. 



Obtained by Mori (1888) from sewer water. 



Morphology. Bacilli with round ends, from 2 to 5 u. long and 0.8 to 1 /* 

 broad. 



Stains with the usual aniline colors, but not by Gram's method ; the 

 ends of the rods are more deeply stained than the central portion. 



Biological Characters. An aerobic, non-liquefying, non-motile bacil- 

 lus. Not observed to form spores. Grows very slowly at the room tempera- 

 ture more rapidly at 37 C. Upon gelatin plates, at the end of two to three 

 weeks, extremely minute, homogeneous, pale-yellow colonies are developed. 

 In gelatin stick cultures a thin, yellowish layer forms upon the surface at 

 the end of three weeks. Upon the surface of agar, at 37 C. , a dry, yellow- 

 ish layer with jagged outlines is developed in two or three days. No growth 

 occurs upon potato. Upon blood serum a thin, pale-green, dry layer is 

 formed. 



Pathogenesis. Mice die in from sixteen to thirty hours after receiving a 

 subcutaneous inoculation, guinea-pigs in about two days. 



137. BACILLUS INDIGOGENUS. 



Obtained by Alvarez (1887) from an infusion of the leaves of the indigo 

 plant. 



Morphology. Bacilli with round ends, about 3 n long and 1. 5 /z thick, 



