NON-PATHOGEXIC BACILLI. 643 



clouded and more or less granular in appearance ; the area of liquefaction 

 increases and the opaque central colony disappears, while the margins of 

 the liquefied gelatin are clouded and whitish. In gelatin stick cultures, at 

 the end of two days, liquefaction has occurred all along the line of puncture 

 and a broad funnel is formed above, while below a mass of white flocculi 

 fills the narrow tube; at the end of three days the gelatin is completely 

 liquefied, it is opalescent, fluorescent, and greenish; on the fifth or sixth 

 day it has a yellowish-green color and a strong odor of putrefaction. Upon 

 the surface of agar, at a temperature of 36 C., it forms circular, grayish- 

 white, almost transparent colonies ; these rapidly coalesce to form a layer of 

 uniform thickness, which is easily broken up. In bouillon the liquid is at 

 first clouded throughout, and at the end of a week has a green color, while 

 the bacilli are seen at the bottom as a pulverulent white deposit. Blood 

 serum is slowly liquefied and gives off a strong putrefactive odor. Upon 

 potato, at the end of forty-eight hours in the incubating oven, a glairy,, 

 grayish layer is formed the size of a five-franc piece ; later this acquires a 

 pale-yellow color. 



294. BACILLUS BUCCALIS MINUTUS. 



Synonym. Bacillus g, Vignal. 



Found by Vignal in the salivary secretions of healthy persons. 



Morphology. A very short bacillus, with round ends, almost as broad 

 as long; in cultures upon agar the length is from 0.5 to 1 /< usually about 

 0.7 n ; in neutral bouillon it is from 1 to 1.7 / long; in old cultures involu- 

 tion forms are common ; in stained preparations the two ends are more deeply 

 stained than the central portion. 



Biological Characters. An aerobic, liquefying, chromogenic bacillus. 

 Produces a yellow pigment. Spore formation not observed. Motility not 

 mentioned. Grows slowly at the room temperature. Upon gelatin plates, 

 at the end of forty-eight hours, the colonies are round, with refractive con- 

 tour and of a mastic-yellow color; they are but slightly elevated and the 

 gelatin commences to liquefy around them. In gelatin stick cultures, at the 

 end of forty-eight hours, a yellowish-white growth is seen along the line of 

 puncture, and upon the surface a layer having the same color and sev- 

 eral millimetres in diameter has developed ; by the fourth day the surface 

 growth has increased to twice the size and is yellow at the centre, while the 

 periphery is white; the growth along the line of puncture is abundant and 

 consists of small, closely crowded colonies; below the surface growth a cup- 

 shaped cavity filled with clouded liquefied gelatin is seen ; by the sixth day 

 a small funnel of liquefaction has formed, the liquefied gelatin is clear and 

 contains some white flocculi in suspension ; by the twelfth day the gelatin 

 in the tube is completely liquefied, an abundant yellow deposit is seen at the 

 bottom and the liquefied gelatin has the same color. Upon the surface of 

 agar golden-yellow plaques are developed, which are easily removed with 

 the platinum needle. In bouillon a thin, iridescent pellicle is formed upon 

 the surface and the fluid below is clouded, while an abundant yellow deposit 

 accumulates at the bottom. Does not grow well in acid bouillon. Upon 

 potato, at the end of forty-eight hours, a thin and extended layer is formed 

 of a yellow color, which later has a brownish tint. 



295. BACILLUS OF CANESTRINI. 



Found in larvae and bees from infected hives in Italy (1891). 



Morphology. Bacilli with rounded ends, from 4 to 6 ^ long and about 

 2 n broad; the isolated elements ai*e somewhat longer than those in chains; 

 solitary, in pairs, or in chains whicli may contain numerous segments. 



Biological Characters. An aerobic, liquefying, motile, chromogenic 

 bacillus. Forms a pink pigment. The movements are slow and oscillating. 

 Forms oval spores 3 /< long and 1.5 /i broad. Grows at the room tempera- 



