G52 NON-PATHOGENIC BACILLI. 



Hue of puncture, and on the surface as a shining layer of moderate extent. 

 Upon agar the growth is similar to that upon gelatin. Upon potato, at the 

 end of thirty-six hours, a dirty-yellow, slimy, granular layer. 



321. BACTERIUM GLISCROGENUM (Malerba). 



Found in urine which was viscid and acid in reaction. 



Morphology. Oval bacilli, from 0.57 to 1.14 u long and 0.41 jit broad. 



Biological Characters. An aerobic, non-liquefying, motile bacillus. 

 Spore formation not observed. Grows at the room temperature in the usual 

 culture media better at 30 to 37 C. Upon gelatin plates, at the end of two 

 days, puuctiform colonies are seen, which gradually increase in size, are per- 

 fectly round, granular, and lenticular in form ; later there is a wavy depres- 

 sion of the surface of the gelatin and gas bubbles are formed in the interior. 

 In gelatin stick cultures growth occurs along the line of puncture and upon 

 the surface in nail shape ; the growth along the line of inoculation consists 

 of disc-shaped, isolated colonies closely piled one upon another. Upon the 

 surface of agar, at the room temperature, a granular, opalescent stripe is 

 developed in from three to five days ; at 37 C. an abundant development 

 occurs in twenty-four hours; a white, viscid film forms upon the surface of 

 the condensation water. Upon potato a yellowish or yellowish-brown layer 

 is developed, and in the course of a few days numerous gas bubbles are 

 formed, which become confluent ; later the growth becomes viscid and ex- 

 tends over the entire surface. In bouillon diffuse cloudiness is seen at the 

 end of twenty-four hours, and the fluid becomes viscid ; at the end of four 

 to five days a whitish layer forms upon the surface. 



322. BACILLUS OVATUS MINUTISSIMUS (Unna). 



Found upon the skin in cases of eczema seborrhoeicum. 



Morphology. Short oval bacilli with pointed ends, 0.6 to 0.8/* long and 

 0.4 // broad; associated in irregular groups. 



Biological Characters. An. aerobic- and facultative anaerobic, non- 

 liquefying bacillus. Spore formation not observed. Grows at the room 

 temperature in the usual culture media. Gelatin and potato cultures give 

 off a strong and disagreeable odor. Updn gelatin plates, at the end of 

 eight days, the superficial colonies are the size of a mustard seed, prominent, 

 spherical, grayish-white, shining, and resemble a drop of a solution of gum 

 tragacanth; the deep colonies are punctiform and grayish- white in color; 

 later the superficial colonies become flat, or occasionally preserve the form 

 of a small pearl ; these are almost one centimetre in diameter, round, finely 

 granular, yellowish-gray, with a darker centre and a more transparent peri- 

 pheral zone; the margins are notched. The deep colonies, under a low 

 power, are seen to be spherical or oval, opaque, finely granular, dark-yel- 

 lowish in the centre and paler at the periphery; they may attain the size of 

 a pea. In gelatin stick cultures growth is rather rapid upon the surface in 

 form of an abundant, slimy, grayish-white layer with irregular outlines; 

 this later becomes dry and opaque, and presents small, spherical protu- 

 berances which are more transparent; along the line of puncture numerous 

 grayish- white, closely crowded, punctiform colonies. Upon a^ar the growth 

 is similar to that upon gelatin. Upon potato an abundant, grayish-white, 

 dull-glistening layer. 



323. CAPSULE BACILLI OP SMITH. 



Theobald Smith has described three species or vai-ieties ( of capsule ba- 

 cilli, resembling Friedlander's bacillus, obtained by him from the intestine of 

 swine. These he designates by the letters a, b, and c. 



Morphology. Slight morphological differences were detected by culti- 

 vating all under the same circumstances in peptone-bouillon . 



