656 NON-PATHOGENIC BACILLI. 



330. BACTERIUM ZOPFH (Kurth). 



Found in the intestine of chickens. 



Morphology. Bacilli from 2 to 5 fj. long and 0.75 to 1 ju broad; form long 

 filaments, which in gelatin cultures are folded and coiled in a peculiar man- 

 ner. In liquid media straight filaments only are seen. The coiled filaments 

 in gelatin cultures form tangled balls, in which they subsequently break up 

 into short rods and finally into spherical bodies which appear to be repro- 

 ductive elements. 



Biological Characters. An aerobic, non-liquefying, actively motile 

 bacillus. Forms spherical spores (?), which are not highly refractive and 

 stain deeply with the aniline colors. Grows best at the room temperature ; 

 at 30 to 37 C. the bacillus is no longer motile ; at 37 to 40 C. involution 

 forms are developed. Upon gelatin plates, at the end of twenty-eight 

 hours, white, punctiform colonies are developed, from which as a centre a 

 mass of fine, radiating filaments is given off; among these numerous small, 

 white points aue distributed, which under the microscope are seen to be 

 spherical zoogloea masses of a brownish-yellow color ; the centre of the colo- 

 nies consists of bundles of interlaced or parallel filaments. In gelatin stick 

 cultures, at the end of twenty-four hours, a thick, white layer is developed 

 along the upper portion of the line of puncture, and later radiating lines of 

 growth are given off from this; these cross each other in various directions 

 and resemble the mycelium of a fungus. No development occurs on blood 

 serum. 



331. BACTERIUM ZURNIANUM (List). 



Found in water. 



Morphology. Short rods with slightly pointed ends, from 1.2 to 1.5 n 

 long and 0.6 to 0.8 /* broad; in stained preparations the ends are most deeply 

 stained, giving the rods the appearance of diplococci. 



Biological Characters. An aerobic, non-liquefying, non-motile bacil- 

 lus. Does not form spores. Grows in the usual culture media at the room 

 temperature better at 25 to 30 C. Upon gelatin plates forms spherical, 

 dirty-white or gray, extremely viscid colonies, which develop into slimy, 

 grape-like masses. In gelatin stick cultures a grape-like mass upon the 

 surface and scanty growth along the line of puncture. Upon potato, at 25 J 

 to 30 C., a slimy, translucent, gray or yellowish-white layer extends over 

 the surface within forty-eight hours. 



332. BACILLUS OF COLOMIATTI. 



Obtained from xerotic masses from the eye of a child and in certain forms 

 of conjunctivitis. 



Morphology. Bacilli which correspond with the bacillus of mouse septi- 

 caemia in length, associated in irregular masses. 



Biological Characters. An aerobic, non-liquefying, non-motile bacillus. 

 Does not grow at the room tempei^ature. Forms spherical spores, which lie 

 at the ends of the rods. Does not grow in nutrient gelatin or on potato. 

 Upon the surface of agar a thin film is developed at 34 to 39 C. , which 

 gives to the surface a fatty lustre. Upon blood serum, at 35 to 39 C., de- 

 velopment occurs along the line of inoculation as a dull-gray layer with a 

 fatty lustre, which has a breadth of two to three millimetres and a rosette- 

 like form. 



333. BACILLUS SCISSUS (Frankland). 



Found in the soil. 



Morphology. A short, thick bacillus of variable dimensions; usually 

 about 1 /LI broad and 1 to 2 it long ; resembles Bacillus prodigiosus, and, like 



