658 NON-PATHOGENIC BACILLI. 



first nail-shaped ; later it spreads out upon the surface. Upon agar a thin, 

 white layer with irregular outlines is developed. In bouillon, at the room 

 temperature, a dense cloudiness is developed and a thick, slimy deposit ac- 

 cumulates at the bottom of the tube. Upon potato a moist, granular, yel- 

 lowish-white layer is developed. In milk an acid reaction is not produced. 



336. BACILLUS PHOSPHORESCENS GELIDUS (Forster). 



Found in phosphorescent sea fish. 



Morphology. In recent cultures the bacilli appear as small rods with 

 slightly rounded ends and about three times as long as broad ; in cultures 

 more than twenty-four hours old they are thicker and nearly oval in form. 



Biological Characters. An aerobic, non-liquefying bacillus. Spore 

 formation not positively determined. Grows slowly at the room tempera- 

 ture, and even as low as 0' C. ; is killed in a few hours by exposure to a 

 temperature of 35 to 37 C. Cultures freely exposed to the air are phos- 

 phorescent in the dark when kept at a temperature between and 20 C. 

 phosphorescence ceases at 32 C. Upon gelatin plates, at the end of forty- 

 eight hours, small, punctiform colonies are developed ; under the microscope 

 these are seen to be spherical, grayish-white in color with a greenish shim- 

 mer ; later granular, yellowish, and with somewhat irregular outlines. In 

 gelatin stick cultures a white layer is developed upon the surface; very 

 scanty growth along the line of puncture. Upon agar the growth is simi- 

 lar to that 011 gelatin. Upon potato a broad, white layer is developed. 



337. BACILLUS SMARAGDINO-PHOSPHORESCENS (Katz). 



Obtained from a herring from the fish market at Sydney, New South 

 Wales. 



Resembles Photobacterium phosphorescens (Cohn) described by Beyer- 

 inck, and Photobacterium Pfliigeri (Ludw.). 



Morphology. Bacilli with somewhat pointed ends, about 2 n long and 

 1 n broad ; solitary or in pairs ; in recent cultures closely resemble cocci. 



Biological Characters. An aerobic, non-liquefying, non-motile bacil- 

 lus. Spore formation not observed. Grows in the usual culture media at 

 the room temperature. Upon gelatin plates, at the end of eighteen hours, 

 the deep colonies are seen as grayish points, and the superficial as thin, 

 grayish- white drops ; under a low power these are pale-gray with a yellow- 

 ish tint, finely granular, and transparent near the margins, which are finely 

 dentate ; diameter from 0.3 to 0.45 millimetre. -The deep colonies, under the 

 microscope, are seen to be oval or lemon-shaped, with a smooth, well-defined 

 contour, and about 0.15 millimetre in diameter; they consist of a broad cen- 

 tral portion surrounded by a narrow central and still narrower marginal 

 zone ; zone formation not observed in colonies in eight-per-cent gelatin. At 

 the end of twenty days the superficial colonies attain a diameter of two milli- 

 metres ; they are flat, have irregular outlines, and are composed of a rela- 

 tively small central portion of a yellowish color, surrounded by a slate-col- 

 ored marginal zone ; the deep colonies at this time (twenty days) are about 

 0.6 millimetre in diameter and yellowish- white in color under the micro- 

 scope straw-yellow. In gelatin stick cultures (six per-cent gelatin) a thin, 

 white line of growth is seen along the line of puncture, and a flat, round, 

 grayish-white layer, with a stearin lustre, is developed upon the surface; this 

 acquires a diameter of about five millimetres. Cultures made by Katz for a 

 year in six-per-cent gelatin gave no evidence of liquefaction, but subse- 

 quently the same bacillus, cultivated in six-per-cent gelatin containing 2.7 

 per cent of sodium chloride, caused liquefaction of the gelatin beneath the 

 surface growth, which gradually extended downward. Growth occurs upon 

 the surface of agar, but this is not a very favorable medium. In neutral 

 bouillon development occurs, and a diffuse cloudiness is seen, but no growth 

 occurs in simple flesh infusion; when, however, 2.5 per cent of sodium 



