724: ADDITIONAL SPECIES OF 



466. UROBACILLUS MADDOXI (Miquel). 



Obtained from sewer water and from river water relatively rare. 



Morphology. Bacilli with round ends, 1 u thick and 3 to 6 ft long ; in 

 old cultures the bacilli are often seen as oval or spherical cells having a 

 diameter of 6 to 8 /* ; in solid media the length is usually from 2 to 3 /*. 



Biological Characters. An aerobic, liquefying, motile bacillus. Forms 

 spores. Grows best at 38 C., but causes fermentation of urine at the end 

 of several days at a temperature as low as 10 C. In nutrient gelatin it 

 usually fails to grow, but occasionally a scanty development occurs along 

 the track of the needle in the form of small, spherical colonies. In nutrient 

 gelatin containing urea the line of inoculation is marked by numerous crys- 

 tals, which are seen at the end of twenty hours, although the growth of the 

 bacilli is scarcely apparent. In gelatin plates containing urea very small, 

 spherical, opaque, whitish colonies, surrounded ^by crystals, may be seen 

 under a low power. In nutrient agar containing urea, at 30 to 35 C., a 

 layer is formed upon the surface, which is at first white and la.ter of a grayish- 

 yellow color. In bouillon which is slightly alkaline it grows rapidly, and 

 produces, at the end of two days, a dense turbidity of the culture liquid ; 

 later an abundant glairy sediment accumulates at the bottom of the tube. 

 Iri one litre of bouillon an amount of soluble ferment is produced by this 

 bacillus which is capable of decomposing sixty to eighty grammes of urea 

 in the course of two or three hours. 



467. UROBACILLUS SCHUTZENBERGI (Miquel). 



Obtained by Miquel from river water and from the water of sewers. 



Morphology. Small oval bacilli about 0. 5 M thick and 1 n long ; usually 

 united in pairs. 



Biological Characters. An aerobic, liquefying, motile bacillus. Spore 

 formation not observed. In nutrient gelatin, at20C., liquefaction in cup 

 shape occurs at the surface and progresses rapidly, being complete by the end 

 of ten days. In gelatin containing two per cent of urea liquefaction com- 

 mences at the surface and numerous crystals are formed in the solid portion 

 of the culture medium ; growth is soon arrested by the antiseptic action of 

 the products developed. In gelatin plates small, translucent, spherical 

 colonies are developed, which increase rapidly in size and acquire a milky 

 appearance; when the colonies reach the surface liquefaction rapidly occurs ; 

 when the gelatin contains urea the colonies cease growing after attaining a 

 diameter of one to two millimetres, and they are surrounded by a zone of 

 crystals. Upon nutrient agar, at 28 to 30 C. , development occurs in the 

 form of a whitish layer with a slightly greenish tint. In bouillon, at the 

 end of twenty-four hours, the medium becotnes clouded ; later a thin pellicle 

 forms upon the surface and extends upward for a short distance upon the 

 walls of the test tube; the bouillon remains clouded for several weeks. 

 When urea is added to peptonized bouillon development is still more abun- 

 dant, but ceases at the end of four or five days and the liquid becomes en- 

 tirely transparent. 



468. BACILLUS OF BOVET. 



Obtained by Bovet (1891) from the intestine of a woman who died of an 

 acute enteritis with choleraic symptoms. 



Morphology. Bacilli from 1 to 1.5 jit thick and 2 to 4 n long, isolated or 

 in pairs. 



Stains with carbol-fuchsin solution. 



Biological Characters. An aerobic, non-liquefying, motile bacillus. 

 Spore formation not observed. Grows in the usual culture media at the 

 room temperature more rapidly at 37 C. In nutrient gelatin, at 15 to 18 

 C., a grayish- white, somewhat translucent layer with irregular outlines is 



