6 BACTERIOLOGY 



Pathogenesis. Dogs inoculated intravenously with 3-4 cc. of culture show 

 symptoms resembling distemper, in other cases keratitis, pyrexia, catarrhal 

 conjunctivitis, etc. 



Habitat. Isolated from the blood in two cases of influenza. 



10. Str. canis 



Ueber einen bei der bakteriologischen Fleischbeschau aufgefundenen Diplococcus. 



Harrevelt: Centralblatt f. Bakteriologie, XXVI, 1899, 121. 

 Morphology. Diplococci, or as chains of 4 elements, rather smaller than Str. 



pyogenes. Stains with ordinary aniline stains. Decolorized by Gram's 



method. 

 Gelatin colonies. No deep colonies. Surface colonies small, similar to those 



of Str. pyogenes, but liquefy the gelatin extremely slowly. Quite flat, dirty 



white, with a brownish centre, round-oval, granular, with a clear border. 

 Gelatin slant. On 8 per cent gelatin a plumose growth along the line of 



inoculation. In 6 days the gelatin is softened, and later entirely liquefied. 

 Agar slant. In 48 hours, at 36 C, a white transparent spreading layer with 



an iridescent lustre. 



Glycerine agar and blood serum. As on agar. 

 Gelatin stab. In 48 hours spreading irregularly over one-third of the surface, 



flat ; liquefaction infundibuliform. 

 Bouillon. Turbid ; slimy sediment. 



Milk. 36, 3 x 24 hours, coagulated ; coagulum slimy ; little acid. 

 Glucose and lactose bouillon. No gas ; rendered acid. 



Starch paste. 36 C., 24 hours, starch liquefied; no sugar, no diastatic ferment. 

 Grows in an acid medium. A slight growth in media containing glycerine. 



Young cultures have an odor of cooked crabs. Reaction of media without 



sugar rendered acid. H,S negative. Indol and nitrites negative. 

 Pathogenesis. Inoculations of rabbits may or may not cause a fatal infection, 



with peritonitis, inflammation of liver, spleen, kidneys, and lymph spaces. 

 Habitat. Isolated from meat. 



ii. Str. coli Escherich 



Sfr. coli-gracilis Escherich : Die Darmbakterien des Sauglings, Stuttgart, 1886. 

 Morphology. .Cocci, 0.2-0.4 /*? usually in S-shaped chains of 6-20 elements. 

 Gelatin colonies. Small, round, becoming punctiform, and lying in funnels of 



liquefied gelatin. 

 Gelatin stab. Liquefaction cylindrical ; sediment white ; gelatin rendered 



acid. 

 Agar slant. A scanty growth. 



