THE CLASSIFICATION OF BACTERIA IOI 



71. M. rugOSUS 



M. No. 2 Conn : I.e., 1893, 50. 



Morphology. Cocci 1.0-1.2 ; never form chains. Grow at 37 C. 



Gelatin colonies. A slight pit of liquefied gelatin becoming coarsely granular 



fragmental. 

 Gelatin stab. Liquefaction shallow crateriform, only one-fourth of the gelatin 



liquefied after several weeks. 



Agar slant. Growth dry, raised, limited, rugose, tenacious, sticky, Naples yellow. 

 Potato. Growth, thick, dry, rough rugose, flesh-colored salmon-brown. 

 Milk. Coagulated, alkaline; butyric acid and alcohol; orange masses on 



the surface. 



Bouillon. Clear, no pellicle, a slight sediment. 

 Habitat. Milk. 



72. M. orbicularis Ravenel 



M. orbicularis-flavus Ravenel : I.e., p. 8. 



Morphology. Cocci large, in irregular groups. Slight growth at 36 C. 



Gelatin colonies. Deep: irregular, finely granular, yellow. Surface: in 3 days 

 minute yellowish dots; in 5 days colonies i mm., round, entire, slightly 

 elevated, becoming crateriform. Microscopically, a homogeneous centre, 

 with granular entire margins. 



Gelatin stab. On the surface a small yellowish button, becoming crateriform, 

 and in 7 days a stratiform liquefaction, with a yellowish flocculent sediment. 



Agar slant. Growth faint yellowish, glistening, limited, becoming canary- 

 yellow. 



Potato. Growth thin, moist, spreading, colorless, becoming thicker and 

 yellow ; then granular, moist, glistening. 



Bouillon. Turbid, whitish sediment, becoming faint yellow. 



Pepton-rosolic acid becomes slightly darker in 2 weeks. 



Litmus milk. Unchanged. 



Glucose bouillon. No gas. Indol slight. 



Nitrites. Negative. 



Habitat. SoilX 



73. M. Tommasoli 



Diplococcus citreus-liquefaciens Unna: Tommasoli, Monatsch. f. prakt. Dermatol., IX, 56. 



Morphology. Cocci small oval, in twos, tetrads, clumps, and short chains. 

 Gelatin colonies . Deep : round-oval, entire, brownish yellow. Surface: in 4 



days, round, flat, grayish white ; in 8 days 1-2 mm. ; in 2 weeks lemon - 



yellow, with crateriform liquefaction. 



