I3 8 BACTERIOLOGY 



Glucose bouillon. No gas, acid. Lactose and saccharose bouillon. No gas, 

 alkaline. Indol produced. Phenol negative. 



Pathogenesis : Fowls inoculated into vein with 0.3 cc. of bouillon culture die in 

 3-13 days ; pyrexia, crouching position, head drawn in, liver slightly 

 enlarged, soft and fatty, spleen rarely enlarged, urates in tubules of 

 kidneys, intestines show punctiform hemorrhagic spots, heart muscles 

 pale with grayish points, only a few bacilli in blood and organs. Rabbits : 

 injections of 0.2 cc. killed in 4-5 days ; slight local reaction, necrotic areas 

 in liver, enlarged and discolored spleen, infiltration of cells into follicles 

 of caecum and glands about ileocaecal valve. 



Habitat. Associated with " infectious leukaemia " in fowls. 



38. Bact. avium 



Bacillus of roup in fowls Moore: U. S. Dept. Ag., Bureau of Animal Industry, 

 Bulletin 8, 1895. 



Morphology. Bacilli 0.8-1.5:0.8-1.2 /m; show polar stain. Decolorized by 



Gram's method ; capsule ( ?) . 

 Agar colonies. Minute grayish dots ; odor pungent, similar to cultures of 



swine plague. 



Agar slant. Growth gray, moist, glistening, slightly viscid. 

 Potato. No growth. 

 Alkaline gelatin. No growth. 

 Milk. Unchanged in 6 weeks. 

 Bouillon. In 24 hours at 36 clouded ; in 2-3 days acid. No growth in acid 



bouillon. 



Glucose bouillon. Acid ; no gas. . , 



Lactose bouillon. Remains alkaline; no gas. Indol produced. Phenol 



negative. 

 Pathogenesis. Injections of o. I cc. subcutaneously into rabbits caused death 



in 18-36 hours with lesions of virulent swine plague. Young fowls 



inoculated with 0.3 cc. subcutaneously died in 4 days; kidneys yellow 



with urates ; cultures from blood and liver positive. 

 Habitat. Isolated from exudate of fowls in " roup " or diphtheria. 



39- Bact. inocuum (Kruse) 



B. lactis-inocuus Kruse : Fliigge, Die Mikroorganismen, 1896, 352. 

 In its morphological and cultural characters similar to Bact. aerogenes. 

 Gelatin colonies. Porcelain-white, round irregular, with characters approach- 

 ing B. colt. 



Potato. Growth brownish. 

 Habitat. Milk. 



