THE CLASSIFICATION OF BACTERIA 159 



Blood serum. Growth dirty white, spreading ; medium liquefied. 



Potato. Slight growth, invisible unless moist, when there form yellowish white 



colonies. 

 Habitat. Isolated from cheese. 



100. Bact. nubilum (Frankland) Lehraann-Neumann. 



B. nubilus Frankland: Zeitsch. f. Hygiene, VI, 1889, 386. 



Bact. nubilum Lehmann-Neumann : Bak. Diagnostik, 1896, 255. 



Morphology. Bacilli slender, 0.3 : 3.0 /A, or filaments more or less curved. 



Gelatin colonies. Like those df Bact. murisepticum. 



Agar slant. Growth thin, opalescent. 



Bouillon. Turbid, with a pellicle. 



Potato. A scarcely visible growth. A slight reduction of nitrates. 



Habitat. Water. 



101. Bact. methylicum (Loew) 



B. methylicus Loew: Centralblatt f. Bakteriol., XII, 1892, 462. 



Morphology. Bacilli short, thick rods, i.o: 2.0-2.5 M- 



Gelatin colonies. In 2 days, round oval, yellowish, entire, sharp, becoming 

 liquefied , edges ciliate. Colonies often like those of cholera. 



Gelatin stab. Liquefaction crateriform, like cholera, with a whitish-yellowish 

 sediment. No liquefaction in depth. 



Glucose gelatin stab. In depth, little or no growth, a small liquefied bubble 

 on top. 



Agar stab. In depth, no growth; on the surface, growth spreading, grayish 

 white. 



Potato. Growth like B. typhosus, pure white, adherent. 



Bouillon. Growth like anthrax; medium clear; on the surface and adherent 

 to the glass, a white ring. Grow well in 0.5 per cent methyl alcohol, 

 0.05 per cent dicalcium phosphate, and o.oi per cent magnesium sulphate, 

 on which it forms a reddish membrane. Possesses the ability to decom- 

 pose formic acid salts. 



Habitat. A culture contamination. 



102. Bact. Trambusti (Kruse) 



Discovered by Trambusti-Galeotti, not named : Centralblatt f. Bakteriol., XI, 1892, 717. 

 B. Trambusti Kruse : Die Mikroorganismen, 1896, 319. 



Morphology. Bacilli 3-5 /x, size variable. Chromatin granules in the interior 



of the rods. Grow at 37. 

 Gelatin colonies. Border irregular, surrounded by a zone of liquefied gelatin. 



