THE CLASSIFICATION OF BACTERIA 183 



CLASS XL WITHOUT ENDOSPORES. OBLIGATE ANAEROBIC. GELATIN 



NOT LIQUEFIED. 



I. Grow at room temperatures and in nutrient gelatin. 



A. Gas produced in ordinary nutrient gelatin. 



1 66. Bact. Welchii Migula. 



B. Gas not produced in ordinary nutrient gelatin. 



1 . Large, thick rods, over i .o micron in diameter. 



167. Bact, emphyscmatosum (Kruse). 



2. Very slender rods, 0.3 micron in diameter. 



1 68. Bact. infecunduni. 



II. No growth at room temperatures or in nutrient gelatin. 



169. Bact. cadaveris Sternberg. 



170. Bact. pyogcnes (Kruse). 



1 66. Bact. Welchii Migula 



Bact. acrogenes-capsulatum Welch-Nuttall : Bull. Johns Hopkins Hospital, July, 1892. 

 Bact. Welchii Migula: System der Bakterien, 1900, II. 



Morphology. Bacilli somewhat thicker than anthrax ; chains with a capsule - 

 Gelatin cultures give much gas. 



Gelatin colonies. 1-2 mm., gray white, oval to irregular with a few out- 

 growths. 



Milk. Coagulated ; has a faint odor of old cheese. 

 Potato. Growth gray white. 

 Pathogencsis negative. 

 Habitat. Isolated from blood in a case of aneurism of the aorta. 



167. Bact. emphysematosum (Kruse) 



B. der Gasphlegmon Fraenkel : Centralblatt f. Bakteriol., XIII, 1893, 13. 

 B. emphysematosus Kruse : Fliigge, Die Mikroorganismen, 1896, 242. 



Morphology. Bacilli somewhat thicker than anthrax ; occur in threads.. 

 Stained by Gram's method. 



Gelatin. With addition of glycerine and formate of soda, active growth with 

 much bad-smelling gas. 



Glucose bouillon. Much gas. 



Bouillon. Turbid. 



Pathogenesis . Guinea pigs, by subcutaneous inoculation, a local non-sup- 

 purating inflammation; later, a necrosis sometimes spreading to perito- 

 neum and pleura. 



Habitat. Isolated from gaseous phlegmon. 



