192 



BACTERIOLOGY 



Agar slant. Growth thin, white, spreading, membranous. 

 Potato. At 37, dirty white, milky colonies, becoming confluent. 

 Pathogenesis. Subcutaneous inoculations of rabbits and guinea pigs show a 



gangrenous process, with death in 2-3 days. 

 Habitat. Isolated from a case of senile gangrene. 



194. Bact. Markusfeldii 



B. der trichorrhexis-nodosa Markusfeld : Centralblatt f. Bakteriol., XX, 1897, 230. 



Morphology. Bacilli 0.4-0.6: 1.7-2.2 /A. Grow at 39. 



Gelatin colonies. Roundish clumps, which quickly liquefy the gelatin. 



Gelatin stab. In 48 hours, a semi-spherical liquefaction of the gelatin. 



Agar colonies. In 24 hours, at 37. Deep colonies : round, oval, white, brown- 

 ish yellow; contour irregular, with ringlet-like outgrowths and small 

 colonies from border. Surface colonies : white, opaque in centre ; border 

 granular to floccose. 



Agar slant. White, moist colonies. 



Milk. Coagulated. 



Potato. Growth moist, white. 



Habitat. Associated with this disease. 



- _ 



195. Bact. crinatum (Wright) 



B. crinatus Wright : I.e., 453. 



Morphology. Bacilli large, chains to segmented threads. 



Gelatin colonies. Deep : dark opaque, round to oval, entire, granular margins. 



Surface: in 2 days, 1-2 mm., round, glistening, translucent, entire; in 



3 days, dense, felt-like margins, crimpled, slightly sunken ; margins also 



fimbriate to frayed. 



Gelatin stab. Liquefaction broadly funnel-formed to stratiform. 

 Agar slant. Growth gray white, frosted. 

 Bouillon. Turbid, with white floculi. 

 Potato. Growth thick, creamy-white, viscid, spreading, becoming yellowish, 



caseous. 



Litmus milk. Decolorized, amphoteric, becoming viscid, yellowish, caseous. 

 Glucose bouillon. No gas. Indol slight or doubtful. Grow at 37. 

 Habitat. Water. 



196. Bact. verticillatum Ravenel 



B. verticillatus Ravenel : I.e., 13. 



Morphology. Bacilli thick, 3-5 times their breadth, rounded. Spores only 

 in potato cultures after 2 weeks. Facultative anaerobic. 



