THE CLASSIFICATION OF BACTERIA 2I/ 



30. B. tracheiphilus Smith 



Centralblatt f. Bakteriol., I, 2d Abt., 1895, 364. 



Morphology. Bacilli 0.5-0.7 : 1.2-2.5 /"* Grow poorly in gelatin. 



Agar slant. Growth thin, smooth, moist, glistening, milky-white, limited. 



Agar stab. Lateral outgrowths from the line of puncture ; on the surface the 



growth is thin. 

 Potato. Growth thin, smooth, moist, glistening ; the color of the potato. Does 



not grow in alkaline media. 

 Bouillon. Turbid cloudy, but not turbid. 



Glucose bouillon. Acid; no gas. Cultures very viscid. No growth at 37. 

 Habitat. Associated with a disease of melons and curcurbits. 



31. B. pinatus Ravenel 



I.e., 32. 



Morphology. Bacilli slender, short, 3-5 times their breadth ; occur singly and 

 in short chains. Motile ; flagella not demonstrated. Facultative anae- 

 robic. 



Gelatin colonies. Deep : round, entire, yellowish, granular. Surface : puncti- 

 form ; do not exceed I mm. ; centre yellowish brown, granular, border clear. 



Gelatin stab. A good growth in depth ; on the surface, growth raised, 2 mm., 

 porcelain-white, umbilicate. 



Agar slant. Growth thin, glistening, watery. 



Potato. Growth thin, colorless to light, dirty brown, smooth, moist, glistening. 



Bouillon. Turbid, with flakes ; no pellicle on the surface. 



Pepton rosolic acid. Decolorized, alkaline. 



Litmus milk. Becomes a darker indigo blue. Indol produced. Nitrates 

 reduced to nitrites. 



Habitat. Soil. 



32. B. Raveneli 



B. geminus-major Ravenel : I.e., 27. 



Morphology. Bacilli thick, ends rounded, of variable length ; occur singly 



and in short chains. Rods show deeply stained points, 2-3 in each rod. 



Motility slight ; flagella not demonstrated. Aerobic. 

 Gelatin colonies. Deep', brownish, granular, entire. Surface: like typhoid, 



but more granular and coarser. 

 Gelatin stab. Delicate offshoots from the line of stab ; on the surface, 



growth thin, spreading, with irregular borders. 

 Agar slant. Growth thin, translucent, spreading. 

 Potato. Growth honey yellow, moist, glistening, becoming chocolate-brown. 



