22 8 BACTERIOLOGY 



Gelatin stab. In depth the growth is beaded, with gas production ; surface 



growth like a gelatin-plate colony. 

 Gelatin slant. Growth white, glistening, waxy, with folded edges, rather 



broad. 



Potato. Growth thick, light yellow, with a granulated surface, raised, 3-4 mm. 

 Bouillon. Turbid, becoming clear, with a heavy white sediment. In urine a 



turbidity, alkaline reaction, and gas, with an aromatic odor. 

 Pathogenesis . Inoculations into dogs result in cystitis. 

 Habitat. Isolated from urine in cystitis. 



65. B. Shigae 



B. of Japanese dysentery Shiga: Centralblatt f. Bakteriol., JXXIII, 1898, 599. 



Morpfwlogy. Bacilli short ; ends rounded. Morphology quite like B. typho- 

 sus, showing involution forms. 



Gelatin colonies. Sharp, yellowish, finely granulated, never very flat like B. 

 typhosus. 



Agar colonies. In 24 hours quite large, round, moist, glistening, brownish, 

 translucent, becoming larger and irregular. 



Potato. Growth scarcely visible, white, dry, becoming reddish brown after 

 some weeks. 



Milk. Not coagulated. 



Glucose bouillon. No gas. Indol not produced. Bacilli show a distinct ag- 

 glutination reaction with serum of persons ill with dysentery, but not 

 with serum of sound men. 



Pathogenesis. Subcutaneous inoculation of guinea pigs gives a strong infiltra- 

 tion at the point of inoculation, with subsequent suppuration. Intraperi- 

 toneal injections cause blood extravasation or peritoneal hemorrhage. 

 Dogs fed on cultures show in 1-2 days slimy stools. Subcutaneous inoc- 

 ulations of man result in chills and fever, headache, etc. The point of 

 inoculation is strongly infiltrated and painful. The serum of man so. 

 treated possesses agglutinating properties. 



Habitat: Isolated from the dejecta of 34 cases of Japanese dysentery. 



66. B. colorabilis Kruse 



B. coli-colorabilis Kruse : Fliigge, Die Mikroorganismen, 1896, 434. 



B. der Gallenblase Naunyn : Deutsch. med. Wochenschrift, 1891, No. 5. 



B. cuniculicida-Havaniensis Sternberg : Manual of Bacteriology, 1892. 

 Morphology. Bacilli short, thick, like Bact. aerogenes, often in twos and short 



filaments. 

 Potato. Growth grayish to yellowish brown. 



