,THE CLASSIFICATION OF BACTERIA 25 1 



1230. B. Rheni 



Rhine water Bacillus (Burri) : See Frankland, Microorganisms of Water, 1894, 483. 

 Morphology. Bacilli 0.7 : 2.5-3.5 /A, ends rounded, sometimes slightly bent. 



In bouillon long filaments and chains. Motility rotatory and progressive. 

 Gelatin colonies. Deep colonies dirty yellow dots, round, entire, granular. 



Surface colonies convex, colorless, transparent, becoming yellowish, with 



an outer zone of clear liquid gelatin. Microscopically, the borders are 



wavy, and contents rough and irregular. 

 Gelatin stab. Growth scanty, in depth ; liquefaction shallow, funnel-formed ; 



yellow flocculent masses in the liquefied gelatin, and a bluish gray opal- 



escence on the surface of the still solid gelatin. 

 Glycerin agar slant. Growth thin, glistening, dry, tough, honey-colored. 

 Potato. Growth moist, glistening, thin, flat ; orange- or rust-colored. 

 Bouillon. Turbid, with a bright, orange-colored pellicle and a yellow deposit. 

 Milk. Coagulated only partially in the upper part of the tube ; the cream 



layer pale yellow ; serum alkaline. No growth at 37. No gas in glucose 



bouillon. 

 Habitat. Rhine River water. 



124. B. aurescens Ravenel 



I.e., 8. 



Morphology. Bacilli short, spindle-shaped, 2-3 times their breadth ; occur 

 singly. 



Gelatin colonies. Surface colonies minute whitish points, which are granular, 

 brownish, and entire. In 4 days 0.5 mm., yellowish brown, homogene- 

 ous, and entire. 



Gelatin stab. Slight growth in depth ; on the surface a yellow button ; in 18- 

 20 days a crateriform dry depression. 



Agar slant. Growth thin, yellowish, limited, becoming golden yellow. 



Potato. Growth yellow, thick, rnoist, spreading, becoming orange-yellow. 



Bouillon,, Slightly turbid, with a dense, flocculent sediment. 



Litmus milk. Reduced, no acid, not coagulated. Indol negative. 



Glucose bouillon. No gas. Grow at 36. 



Habitat. Soil. 



125. B. cuticularis Tils 



Zeitsch. f. Hygiene, IX, 1890, 282. 



Morphology. Bacilli 0.3-0.5 : 2.0-3.0 //, filaments. 



Gelatin colonies. Coli-like. The deep colonies are brown, irregular entire; 

 the surface colonies have brown centres and colorless borders. 





