2/0 



BACTERIOLOGY 



Agar slant. Growth thin, greenish white, translucent. 



Gelatin stab. Slight growth in depth, with later spherical outgrowths ; sur- 

 face growth capitate, sunken in the gelatin ; evaporation exceeds lique- 

 faction. 



Potato. In 3 days, a bluish discoloration along the line of inoculation, 

 becoming yellowish, moist, glistening. 



Bouillon. A slight turbidity, becoming clear. 



Pepton rosolic acid solution. Decolorized. 



Litmus milk. Alkaline, not coagulated. In two weeks becomes translucent 

 and violet. 



Glucose bouillon. No gas. Indol negative. Optimum temperature 36. 



Habitat. Soil. 



170. B. alpha Dyar 



I.e., 366. 



Morphology. Bacilli large, 0.8 : 1-2 /*; occur singly and in chains. 



Gelatin. Not liquefied for 1 1 days or more. 



Agar slant. Growth translucent, glistening, white. 



Litmus milk. Not coagulated ; not reddened. Reduction of nitrates negative 



or slight. Indol production slight. 

 Habitat. Air. 



171. B. beta Dyar 



I.e., 366. 



Morphology. Bacilli short, rounded, 0.6: 1.5-2.0 /u,. 



Gelatin. Liquefied slowly. Surface : growth thin, feathery. 



Agar slant. Growth translucent, glistening, white. 



Litmus milk. Not coagulated ; not reddened. Reduction of nitrates negative 



or slight. Indol production slight. 

 Habitat. Air. 



172. B. Pammelii 



B.-gasoformans Pammel : Centralblatt f. Bakteriol., 2te Abt., II, 1896, 642. 



Morphology. Bacilli 1.2-1.4:3.7-4 /x, variable; in chains of 2-5. Motility 



slight. 



Gelatin colonies-. Lenticular. 

 Gelatin slant. Growth white, limited. 

 Gelatin slab. Liquefaction infundibuliform. 

 Agar slant. Growth white, wrinkled. 

 Bouillon. Good growth, with a pellicle. 

 Potato. Slightly granular, large wrinkles, spreading. 



