THE CLASSIFICATION OF BACTERIA 2/5 



Bouillon. Turbid, with a slight pellicle. 



Potato. Growth spreading, light brown, reticulately wrinkled. 

 Litmus milk. Decolorized, amphoteric, coagulated. 



Glucose bouillon. No gas. Indol production slight or doubtful. Grow at 36. 

 .Habitat. Water. 



1 88. B. maidis Paltauf-Heider 



Wiener Med. Jahrb., 1888, 383. 



Not differentiated from B. uulgatus^ except potato growth is wrinkled, and 



yellow-brown. 

 Habitat. Isolated from spoiled corn. 



189. B. plicatus 



B. lactis No. 7 Flugge : Zeitsch. f. Hygiene, XVII, 1894. 294. 



.Morphology. Bacilli long. 



Agar slant and potato. Growth brown, crumpled. 



Bouillon. Turbid, slight pellicle. 



Milk. Peptonized, toxic. 



Habitat. Milk. 



190. B. gangraenae Arkovy 



B.gangrcence-pulpcB Arkovy: Centralblatt f. Bakteriol.. XXIII., 1897, 921. 



Morphology. Bacilli 4.0 /x, square-ended ; occur singly and in chains. Occa- 

 sionally as filaments or coccoid forms, with other degeneration forms in 

 old agar cultures. Stain by Gram's method. Spores oval, in the centre 

 of the rods. 



Gelatin colonies. In 24 hours, white, mealy. Microscopically, finely granular, 

 golden yellow, round, with outgrowths; in about 30 hours the gelatin is 

 liquefied ; on the fluid gelatin a white, crumpled membrane ; a stinking, 

 cheesy odor. 



Gelatin stab. In 24 hours a small funnel with a white membrane; liquefaction 

 extending to walls of the tube. The membrane becomes dirty brown, the 

 gelatin reddish brown and alkaline. 



Agar colonies. In 24-30 hours white, mealy, like gelatin colonies. 



Agar slant. Growth limited, crumpled, becoming in 5-6 days ash-gray ; the 

 medium a beautiful brownish gray. 



Blood serum. A brownish stripe, liquefied. 



Bouillon. A membrane ; medium-colored, like the liquefied gelatin. 



Potato. Growth moist, brown, crumpled. 



Milk. Coagulated. 



