THE CLASSIFICATION OF BACTERIA 289 



Litmus milk. Color discharged in 12 days, alkaline, not coagulated. 

 Glucose bouillon. No gas. Indol negative. Grow at 36. 

 Habitat. Soil. 



229. B. saprogenes Kramer 



B. saprogenes-vini No. VI Kramer : Bakteriologie Landwirtsch., 1890, 139. 



Morphology. Bacilli i.o : 2.0 /x. Obligate aerobic. 



Gelatin stab. Surface growth dirty white ; liquefied rapidly. Ammonia 



developed in old cultures. 

 Habitat. Isolated from diseased wine. 



230. B. Baccarinii Macchiati 



Centralblatt f. Bakteriol., IV, 1898, 332. 



Morphology. In gelatin cultures rods 0.7-0.8:2.0-3.5 /tx, straight slightly 

 curved; occur singly, in chains, and as zobgloea. In bouillon, long 

 filaments like Leptothrix. Flagella peritrichic. Rods swollen in the 

 middle at sporulation, oval. Spore germination polar. 



Gelatin stab. In 4-5 days, an empty shallow funnel, beginning with an air 

 bubble above, lined with a white growth, with a membrane on the lique- 

 fied gelatin. The gelatin of old cultures is colored black, and is fluores- 

 cent. 



Agar slant. Raised, yellowish colonies, becoming gray, spreading. 



Milk. In 3-4 days, coagulated ; in 14 days, peptonized, acid. 



Potato. Growth light yellow, becoming straw yellow; potato liquefied or 

 softened. Optimum temperature 23-25, maximum 40. 



Habitat. Associated with mal-nero of the vine. 



231. B. Duclauxi Miquel 



Urolacillus Duclauxi Miquel: Ann. Micrographie, II, 1889, 58. 

 Morphology. Bacilli 0.6-0.8 : 2-10 //,; chains. Gelatin slowly softened. 

 Bouillon (made alkaline with NH 4 HO). Turbid, becoming viscous ; bad odor. 

 Habitat. Isolated from canal and river water. 



232. B. cinctus Ravenel : I.e. 

 Morphology. Bacilli straight, variable, involution forms. Rods show deeply 



stained spots. 



Gelatin colonies. In 24 hours, minute, yellowish, granular, entire ; in 72 hours, 

 0.5 mm., nucleus surrounded by a yellowish zone, then a gray-veined 

 zone, with irregular margins. In 8 days, i mm., grayish white, round,- 

 elevated, entire, finely veined mottled, often with ferny outgrowths, 

 u 



