THE CLASSIFICATION OF BACTERIA 299 



255. B. cuneatus 



B. Anaerobic No. Ill Fliigge : Zeitsch. f. Hygiene, XVII, 1894, 272. 



Morphology. Bacilli long ; cuneate at sporulation. 



Gelatin colonies. Yellow brown ; contour sharp ; border irregular. 



Agar colonies. Dark brown, irregular, lacerate. 



Glucose bouillon. Turbid; gas; rancid odor. 



Milk. Unaltered in 8 days. 



Habitat. Milk. 



256. B. sporogenes Klein 



B. enteritidis-sporogenes Klein: Centralblatt f. Bakteriol., XVIII, 1895, 737, XXII, 578, 

 XXIII, 542,913. Report Loc. Gov. Board, Supplement, 1897-1898, 210. 



Morphology. Bacilli 0.8 : i .6-4.8 /u, filaments chains. Stained by Gram's 



method. Form oval spores, mostly polar, with clavate enlargement of 



the rod. Flagella mostly at one side of an end, in bundles. 

 Glucose gelatin colonies. Uniform, spherical, finely granular, translucent 



masses of liquefied gelatin. 

 Agar colonies. Round, gray disks, quite opaque in centre and granular at 



border. 

 Blood serum. Growth thin, gray ; medium liquefied.; fluid turbid, alkaline, 



and stinking. 



Glucose bouillon. Turbid ; often no growth. 

 Potato. Anaerobically, at first no growth; in 8-14 days, a number of small 



round yellowish colonies. 

 Formate of soda agar colonies. Small, flat, gray, with dark granular centres 



and clear sharp borders. Grow best in milk, becomes translucent, pep- 



tonized, with much gas. 

 Pathogenesis . Virulent cultures become greatly attenuated in 3-4 generations. 



Subcutaneous inoculations of i cc. of a milk culture into guinea pigs 



cause death in 18-24 hours. Strong oedema, muscular tissue strongly 



infiltrated, and a bloody stinking cedematous fluid. Bacilli sparingly 



present in heart, blood, and spleen. 

 Habitat. Widely distributed sewage, water, horse and cow manure, street 



dust. 



257. B. spinosus Ltideritz 



Zeitsch. f. Hygiene, V , 1888, 152. 



Morphology. Bacilli oi6 : 4-8 /x. Spores at end of rod where the latter is 

 swollen. No starch reaction with iodine. 



