302 BACTERIOLOGY 



CLASS XXII. WITH ENDOSPORES. OBLIGATE ANAEROBIC. RODS 

 SWOLLEN AT ONE END AT SPORULATION. 



TETANUS GROUP. 

 I. Gelatin liquefied. 



A. Gelatin stab cultures arborescent or with radiating outgrowths. 



263. B. tetani Fliigge. 



264. B. pseudoletanicMs Sanfelice. 



B. Gelatin stab cultures not at all arborescent. 



265. B. cuneatus. 

 II. Gelatin not liquefied. 



A. Anaerobic at room temperatures. 



266. B. Lubinskii Kruse. 



267. B. longus. 



B. Anaerobic only at body temperatures. Will grow with access of air 



at room temperatures. Non-pathogenic. 



268. B. pseudotetanicus var. aerobius Kruse. 



III. Do not grow in gelatin ; at least cultures therein unsuccessful. 



269. B. Ta-ueli. 



263. B. tetani Fliigge 



Tetanus Bacillus Nicolaier: Deutsche med. Wochensch., 1884, No. 52. 

 B. tetani Fliigge : Die Mikroorganismen, 1886. 

 Pacinia Nicolaieri Trevisan : Genera, 1889. 



Morphology. Bacilli 0.3-0.5 : 2-4 /z filaments. Slightly motile. Flagella 



peritrichic. Stain by Gram's method. 

 Gelatin colonies. Small, white, punctiform, becoming sunken and surrounded 



by a zone of liquefied gelatin ; microscopically, the centres are yellow 



brown ; borders floccose fragmented. 



Gelatin stab. Growth arborescent, slowly liquefied, with some gas. 

 Agar stab. A fir tree growth. 

 Bouillon. A uniform turbidity. 



Milk. ' Not coagulated, amphoteric. H 2 S positive. Indol slight. 

 Glucose bouillon. Gas. 



Pathogenic. To mice, guinea pigs, rabbits, horses, etc. 

 Habitat. Associated with tetanus. 



264. B. pseudotetanicus Sanfelice 



Zeitsch. f. Hygiene, XIV, 1893, 372. 

 Morphological and cultural characters like the preceding, only differs in its less 



toxic properties. 

 Habitat. Isolated from meat infusion and earth. 



