322 



BACTERIOLOGY 



Habitat. In the mouth, intestines, and on the surface of the body ; in sup- 

 purating wounds ; sometimes associated with peritonitis, appendicitis, in 

 phlegmons, otitis media, bronchopneumonia, etc. 



VARIETIES (non-pathogenic). B. fluorescens-mutabilis Wright : I.e., 449. | 

 Habitat, water. 



B. No. 21 Conn.: I.e., 1893, 52. Habitat, milk. 



These are probably only non-pathogenic varieties of the preceding, except 

 that the chromogenic function is weaker. Cultural characters otherwise | 

 within the range of probable normal variations. 



29. Ps. capsulata (Pottien) 



B. fluorescens-capsulatus Pottien : Zeitsch. f. Hygiene, XXII, 1896, 146. 



Morphology. Bacilli small, with rounded ends, scarcely one-half the length of 

 tubercle bacilli, and somewhat thicker ; often in twos, somewhat variable 

 in size, rarely coccoid. Show a polar stain. Not stained by Gram's 

 method. A capsule in the body and on media. A long, undulating polar 

 flagellum. Optimum temperature 37. 



Gelatin colonies. Deep : grayish, greenish, concentric, often with radiate 

 fibrils. Surface : brownish, granular, becoming liquefied in 4 days ; a 

 greenish shimmer, with sunken surface growth ; microscopically, concen- 

 tric, the outer zone has a B. subtilis character. An odor in the liquefied 

 gelatin of musty cheese. 



Gelatin stab. Liquefaction saccate infundibuliform, becoming stratiform ; 

 the liquid gelatin becomes yellowish green bluish red. 



Agar slant. Growth moist, glistening, slimy ; a bluish shimmer, with a gray 

 green blue green fluorescence. 



Bouillon. A thick, slimy membrane, and a green fluorescence. 



Glucose bouillon. No gas. 



Milk. '37, a green fluorescence at the surface; at 20, coagulated in 48 

 hours. 



Potato. Growth grayish green yellowish green, slimy. Grow in acid gelatin. 

 Indol positive or scanty. Reduction of nitrates to nitrites negative or 

 scanty. 



Pathogenesis . Subcutaneous inoculation of small doses into mice cause death 

 in 1-3 days, with cramps, oedema at the point of inoculation, and inflam-' 

 mation of the internal tissues. Bacilli by culture from blood and organs. 



Habitat. Isolated from a case of cholera-nostras. 



