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BACTERIOLOGY 



Gelatin stab. In depth, growth thick, granular, yellow ; on the surface, a yel- 

 lowish expansion, with a bowl-shaped depression. 



Agar slant. Growth dirty white, becoming thicker, golden yellow, pasty. 

 Potato. Growth thick, pasty, golden yellow. 

 Bouillon. Turbid ; no pellicle. 

 Habitat. Sewer mud. 



MICROSPIRA Migula 



Cells mostly small, weakly curved comma forms or short spirals, occasionally 

 longer spiral filaments, which, with iodine staining, may show segmenta- 

 tion into comma elements. Every cell bears, as a rule, one polar flagel- 

 lum, and, less commonly, 2-3. Immediately before division there may be 

 flagella at both poles. Endospores not known. 



I. Cultures show a bluish to a silvery phosphorescence. 



MARINE BACTERIA. 



1. Microspira phosphorescens (Fischer). 



2. Microspira Fischeri (Beijerinck). 



3. Microspira luminosa (Beijerinck). 

 II. Cultures do not show phosphorescent properties. 



A. Gelatin liquefied. 



1. Cultures show the nitro-indol reaction. 



a. Very pathogenic to pigeons. 



4. Microspira Metschnikovi (Gamalei'a) Migula. 



5. Microspira Schuylkilliensis (Abbott). 



b. Not distinctly pathogenic to pigeons. 



* Milk coagulated. 



f Gelatin liquefied rather slowly. 



6. Microspira comma (Koch) Schroter. 

 ft Gelatin liquefied rapidly. 



7. Microspira danubica (Heider). 

 ** Milk not coagulated. 



8. Microspira Berolinensis (Neisser) Migula. 



2. Nitro-indol reaction negative or very weak, at least after 24 hours. 



a. . Grow on potato and in neutral bouillon. 



9. Microspira protea. 



10. Microspira Gindha (Kruse). 

 b. No growth on potato. 



* Liquefaction of the gelatin takes place only at the surface, 



crate riform. 



11. Microspira aquatilis (Giinther). 



